重组抗原rSjPGM和rSjRAD23用于检测山羊日本血吸虫病的初步研究  

Primary study of detecting goat schistosomiasis japonica using recombinant antigens rSjPGM and rSjRAD23

作　　者：吕超[1,2] 洪炀[2] 傅志强[2] 陆珂[2] 曹晓丹[2] 王涛[2] 贾秉光 韩倩[2] 朱传刚[2] 李浩[2] 林矫矫[2] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234 [2]中国农业科学院上海兽医研究所农业部动物寄生虫学重点开放实验室,上海200241

出　　处：《中国兽医科学》2016年第2期232-236,共5页Chinese Veterinary Science

基　　金：国家自然科学基金资助项目(31172315)

摘　　要：以日本血吸虫基因重组抗原SjPGM(rSjPGM)和SjRAD23(rSjRAD23)作为诊断抗原,以日本血吸虫虫卵抗原(SEA)作为对照抗原,应用间接ELISA方法对91份日本血吸虫感染山羊血清、44份健康山羊血清、12份捻转血矛线虫感染山羊血清进行了检测。结果显示,rSjPGM和rSjRAD23的敏感性分别为81.3%和66.0%,特异性均为100%,和捻转血矛线虫病血清均无交叉反应;把两种抗原等量混合后检测的敏感性提高到91.2%,特异性下降至95.5%,和捻转血矛线虫感染山羊血清无交叉反应;以SEA作为诊断抗原的敏感性为100%、但特异性仅为75.0%,并且和山羊捻转血矛线虫病血清有25.0%的交叉反应。结果表明,rSjPGM和rSjRAD23混合抗原可作为诊断抗原,用于山羊血吸虫病诊断。The purified recombinant proteins SjPGM（rSjPGM） and SjRAD23（rSjRAD23） of Schistosoma japonicum were employed as diagnostic antigens for detecting goat schistosomiasis japonica by ELISA and used soluble egg antigens（SEA） of S.japonicum as a control antigen. A total of 91 schistosome in fected goat sera,44 noninfected goat sera and 12 Haemonchus contortus infected goat sera were detected.The results showed that the sensitivity of rSjPGM and rSjRAD23 was 81.3% and 66.0%,the specificity of the both 100%,and there was no cross reaction with H.contortus infected sera.The sensitivity was increased to 91.2% and the specificity was reduced to 95.5% when the two antigens were mixed with equal amounts and used as diagnostic antigens. In contrast,the sensitivity,specificity and cross reaction rate of SEA was 100%,75.0%,and 25.0%,respectively.The primary study suggested that equal mixed antigens of rSjPGM and rSjRAD23 can be used as potential diagnostic antigens for detecting goat schistosomiasis japonica.

关 键 词：基因重组抗原rSjPGM和rSjRAD23 诊断 山羊 日本血吸虫病 

分 类 号：S852.735[农业科学—基础兽医学]