大鲵补体C1q及其补体信号通路相关基因的生物信息学分析  

In silico analysis of complement C1q and complement signaling related genes from the Chinese giant salamander(Andrias davidianus)

作　　者：张启焕[1] 齐志涛[1,2] 乔帼[2] 王资生[1,2] 吕富[2] 王普泽[2] 邵荣[1,2] 

机构地区：[1]江苏省海洋滩涂生物化学与生物技术重点建设实验室,盐城工学院,江苏盐城224051 [2]江苏省沿海池塘养殖生态重点实验室,盐城工学院,江苏盐城224051

出　　处：《生物技术》2016年第1期53-57,共5页Biotechnology

基　　金：国家自然科学基金项目(“大鲵肽聚糖识别蛋白家族及其抗菌免疫功能研究”,No.31302221)资助

摘　　要：[目的]对大鲵补体C1q及其补体信号通路相关基因进行生物信息学分析。[方法]利用BLAST软件从大鲵肝脏转录组中筛选获得其补体C1qcDNA全长，并进行了序列比对、系统进化树及3-D结构分析。对大鲵补体信号通路相关基因进行搜索并构建信号传导通路。[结果]大鲵补体C1qcDNA全长为990bp，编码246个氨基酸。大鲵补体C1q与其他物种C1q的序列相似性为32．9％～73．4％。一系列补体系统相关基因在大鲵中被发现，包括补体C1r、C1s、C2、C3、CA、C7、C8、C9以及补体调节蛋白等。[结论]获得了大鲵补体C1qcDNA序列及其信号传导通路基因（C1rxC1s、C2、C3、C4、C7、C8、C9）及调节蛋白，为深入研究两栖类乃至低等脊椎动物的补体系统功能奠定了基础。[ Objective ] To bioinformatic analyze the complement C1 q and genes related with complement signaling pathway of Chinese giant salamander. [ Methods] The transeriptome of liver of salamander were searched by BLAST software to obtain the full cDNA length of complement C1q and sequence alignment, phylogenetic tree and 3 -D structure were further analyzed. In addition,the genes involved in the complement signaling pathway were obtained by searching the salamander liver transcrip- tome. [ Results ] The salamander C1 q cDNA was 999 bp in length, encoding 246 amino acids. Salamander C1 q shared 32.9% - 73.4% sequence similarities with C1q of other vertebrates. A number of genes involved in the complement signaling pathway were obtained, such as complement Clr, Cls, C2, C3, C4, C7, C8, C9 and complement regulatory proteins. [ Conclusion ] The complement C1 q, Clr, C 1 s, C2, C3, C4, C7, C8, C9 and some complement regulatory proteins were obtained in the Chinese giant salamander,which provide the basis for further studying the function of complement system in salamander and further in amphibian.

关 键 词：大鲵 补体 C1Q 信号通路 生物信息学 

分 类 号：Q811.4[生物学—生物工程]