T-2毒素诱导人结肠癌细胞凋亡的实验研究  

Experiment research of T-2 toxin on apoptosis in human colon cancer

作　　者：刘磊[1] 冯淳[1] 朱文赫[2] 董媛[1] 王会岩[1] 

机构地区：[1]吉林医药学院检验学院,吉林吉林132013 [2]吉林医药学院基础医学院,吉林吉林132013

出　　处：《生物技术》2016年第1期98-102,共5页Biotechnology

基　　金：国家科技重大专项("T-2烯霉素注射液的研制";No.2012ZX09103-301-003);吉林省教育厅"十二五"科学技术研究项目("T-2毒素的纯化及对肿瘤细胞作用的研究";No.2013-355);吉林省大学生创新创业训练计划项目("T-2毒素酶联免疫吸附(ELISA)检测方法的研究";No.2015023)资助

摘　　要：[目的]研究T-2毒素对人结肠癌细胞SW115的增殖抑制与凋亡作用。[方法]体外培养人结肠癌细胞SW115,加入浓度为1.6、8、40、200、1 000μg/m L的T-2毒素作用24 h,MTT法检测细胞增殖抑制率,流式细胞仪检测细胞凋亡,Hoechest 33258染色法观察细胞凋亡形态,并检测Caspase-3的活性变化。[结果]与对照组相比,T-2毒素为40μg/m L时显著抑制SW115细胞增殖(P<0.01),当T-2毒素达到200μg/m L,流式细胞仪检测凋亡率为27.55%,细胞内Caspase-3活性为0.597,差异具有统计学意义(P<0.01)。[结论]T-2毒素对人结肠癌细胞SW115具有明显的抑制作用,具有诱导SW115细胞凋亡的作用。[ Objective] Inhibitory and apoptosis effects was investigated with T- 2 toxin on human colon cancer SWl15. [ Methods] The SW115 cells cultured in vitro cells were incubated with T- 2 toxin at the concentration of 1.6,8,40,200 and 1 000 μg/mL for 24 hours. The proliferation of SW115 were examined by MTT cycle and apoptosis analysed by flow cytom- ctry, Hochest 33258 staining was used to observe of cell morphology, the activity change of Caspase -3 were detected. [ Re- suits] Compared with the normal control group, the proliferation of SW115 was significantly inhibited by T -2 toxin when the concentration of 40 μg/mL（ P 〈 0.01 ）. The apoptosis rate was 27.55 % by flowcytometry （FCM） when T -2 toxin reached 200 μg/mL, and the Caspase -3 activity was increased 0. 597 which was significantly different from the control group （P 〈 0.01 ）. [ Conclusion] Human colon cancer SW115 cells was significantly inhibited and induced apoptosis by T- 2 toxin.

关 键 词：T-2毒素 SW115 细胞凋亡 CASPASE-3 

分 类 号：Q279[生物学—细胞生物学]