新疆额尔齐斯河流域杨属植物种间关系的SSR分析  

Phylogenetic Relationship Analysis of Populus along Erqis River

作　　者：殷继艳[1,2] 张建国[1] 何彩云[1] 保尔江 段爱国[1] 曾艳飞[1] 王健 

机构地区：[1]中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京100091 [2]中国武警警种学院,北京102202 [3]阿勒泰地区林业科学研究所,新疆阿勒泰836500

出　　处：《林业科学研究》2016年第1期17-24,共8页Forest Research

基　　金：中央公益性科研院所基本科研业务费专项资金项目“额尔齐斯河天然杨树种群遗传多样性研究”(201004035)

摘　　要：[目的]为探讨额河杨和银灰杨天然杂种的起源机制,[方法]应用18对SSR标记,从分子水平上对新疆额尔齐斯河流域杨属植物的种间关系进行分析研究。[结果]表明:(1)SSR系统发育树将整个流域天然杨属植物分为两大类群,即黑杨派和青杨派为一类,白杨派为一类;(2)白杨派派内系统聚类图显示,银白杨、欧洲山杨、银灰杨三个树种均有较大的遗传分化,特别是杂种银灰杨似乎更大;(3)黑杨派和青杨派的UPGMA分类图显示,青杨派和黑杨派分属于2个分支,其中,青杨派内部分化相对简单,分为2支,均为典型的苦杨;黑杨派内部的分化较为复杂,可分为4类,包括典型的欧洲黑杨、额河杨和回交子代。[结论]杂种额河杨具有更多的欧洲黑杨的遗传成分,因此,将额河杨放到黑杨派是正确的。To explore the hybrid origin mechanism of Populus × jrtyschensis and P. canescens,the phylogenetic tree based on nuclear microsatellite markers（ SSR） analysis was structured. The result show that the natural Populus populations can be classified into two groups,the one is Section Leuce,and the other is Section Aigeiros and Section Tacamahaca. In Section Leuce,significant genetic differentiation was found in the populations of Populus alba,P. canescens and P. tremula,especially the crossbreed Populus（ P. canescens）. A UPGMA phylogenetic tree shows that Section Aigeiros and Section Tacamahaca can be divided into two groups. Compared with Section Tacamahaca,the genetic differentiation in Section Aigeiros is more complex,and they can be further divided into four subgroups,including P. nigra,P. × jrtyschensis and their backcross progeny. It is confirmed that P. × jrtyschensis should be classified as Section Aigeiros based on their smaller genetic distance.

关 键 词：额尔齐斯河 杨属 种间关系 SSR 

分 类 号：S792.11[农业科学—林木遗传育种]