阿勒泰羊不同繁殖状态下丘脑Kiss1基因表达规律与其季节性繁殖的关联性分析  

Correlation Analysis of the Expression Rules of Kiss1 Gene in Hypothalamus of Altay Sheep in Different Reproduction Status and Its Seasonal Reproduction

作　　者：张伟[1,2,3] 刘晓娜[1] 邓双义[1] 王世银[1] 石国庆[2,3] 

机构地区：[1]新疆农业职业技术学院,昌吉831100 [2]新疆农垦科学院畜牧兽医研究所,石河子832000 [3]石河子大学动物科技学院,石河子832000

出　　处：《基因组学与应用生物学》2016年第1期45-52,共8页Genomics and Applied Biology

基　　金：新疆农业职业技术学院科研项目(XJNZYKJ2013008);国家自然科学基金(31360540);国家科技支撑计划(2011BAD28B05-1-1)共同资助

摘　　要：为了研究Kiss1基因在阿勒泰羊中的组织表达情况,及其在不同发情状态阿勒泰羊下丘脑中表达量的变化,本研究探讨其在阿勒泰羊季节性发情调控中的作用机理。本研究以季节性发情的阿勒泰羊为研究对象,首先使用半定量RT-PCR对发情盛期阿勒泰羊下丘脑、垂体、卵巢、心肌等10个组织中Kiss1基因的表达情况进行了检测,并使用q RT-PCR技术对发情前期、发情盛期、发情末期和间情期状态阿勒泰羊下丘脑中Kiss1基因的表达变化进行研究。结果表明,Kiss1基因在发情盛期阿勒泰羊的下丘脑中高表达,在垂体、卵巢和甲状腺中表达量相对较低,在心、肝、脾、肺、肾和肌肉中不表达。Kiss1基因在乏情期(夏至日前后)阿勒泰羊下丘脑中的表达量很低,至发情前期下丘脑中Kiss1基因的表达量迅速上升,并达到峰值,极显著高于乏情期和间情期(p<0.01)。发情启动后,下丘脑中Kiss1基因的表达量逐渐下降,其中发情盛期和发情末期的表达量之间差异不显著(p>0.05),但是显著高于间情期(p<0.05),极显著高于乏情期(p<0.01),间情期的表达量又显著高于乏情期(p<0.05)。以上数据提示Kiss1基因可能主要在诱导阿勒泰羊发情启动阶段发挥重要作用。To investigate expression of Kiss 1 gene in main tissue of Altay sheep, and expression variations in hypothalamus of Altay sheep in different estrous states, this study explored the mechanism of Kissl gene regulation in seasonal estrus control of Altay sheep. This study chose the Altay sheep which is seasonal estrous sheep as the research model. First of all, the expression ofKissl gene in ten tissues （hypothalamus, pituitary, ovary, heart, etc） of Altay sheep in the peak estrous stage and its expression variations in hypothalamus of initial estrous stage, peak estrous stage, final estrous stage and anoestrus stage were detected by using RT-PCR and qRT-PCR. The result showed that Kissl gene expressed in hypothalamus at a high level in the peak estrous stage, and at a relatively low level in pituitary, ovary and thyroid organ, and skeletal muscle. The expression levels ofKissl gene but we did not detect in heart, liver, spleen, lung, kidney in hypothalamus of Altay sheep during anoestrus season （before or after summer solstice） were very low, but when they entered initial estrous season, the expression levels ofKissl gene rose quickly and reached a top level. The expression levels ofKissl gene were extremely high thananoestrus season and diestrus （p〈0.01）. When estrous started, the expression levels ofKissl gene in hypothalamus gradually fell. There were not significantly different between peak estrous stage and final estrous stage （p〉0.05）, but they were relatively higher than diestrus stages （p〈0.05）, and significantly high than anoestrus （p〈0.01）, and diestrus stage were higher than anoestrus （p〈0.05）. The above results indicated that Kissl gene may play an important role in turning on the estrous of Altay sheep.

关 键 词：阿勒泰羊 下丘脑 Kiss1基因 季节性发情 

分 类 号：S826[农业科学—畜牧学]