哺乳动物体细胞核移植重编程研究进展  

Research Progress on Reprogramming of Somatic Cells Nuclear Transfer in Mammals

作　　者：曹慧[1] 苏文龙[1] 倪俊卿[2] 马亚宾[2] 李俊杰[3,4] 

机构地区：[1]河北农业大学动物科技学院,保定071000 [2]河北省畜牧良种工作站,石家庄050061 [3]河北农业大学动物医学院,保定071000 [4]河北省牛羊胚胎工程技术研究中心,保定071000

出　　处：《基因组学与应用生物学》2016年第1期100-106,共7页Genomics and Applied Biology

基　　金：河北省自然科学基金(C2014204119);河北省高等学校科学技术研究项目(ZD2014002);河北省奶牛产业体系共同资助

摘　　要：哺乳动物通过体细胞核移植技术能够获得重构胚,并在卵母细胞重编程作用下,供体细胞恢复全能性,这即是核重编程过程。但由于异常重编程的存在,常会导致克隆后代出现高流产率、高死亡率和发育异常等现象。因此,本文主要从细胞分化对重编程的影响、早期胚胎的重编程能力、重编程的检测方法以及提高体细胞核移植重编程的方式这几个方面加以综述,以期进一步增强对体细胞核移植重编程的认识,完善体细胞核移植技术程序。Mammalian reconstructed embryos can be obtained by somatic cell nuclear transfer, and the totip- otency of donor cells will be recovered under the influence of oocyte reprogramming, which is the process of nuclear reprogramming. However, high rate of abortion, mortality and dysplasia in the clonal offspring are often existed because of abnormal reprogramming. Thus, we reviewed the effect of cell differentiation on reprogr- amming, the ability of early embryos reprogramming, the detection methods of reprogramming and the way to improve reprogramming of somatic cell nuclear transfer in order to enhance the understanding of nuclear reprogr- amming further and optimize the procedure of somatic cell nuclear transfer.

关 键 词：体细胞核移植 重编程 DNA甲基化 组蛋白乙酰化 

分 类 号：Q813[生物学—生物工程]