蜘蛛香成分11-ethoxyviburtinal对肠易激综合征大鼠肠嗜铬细胞及肥大细胞表达的影响  

Influences of 11-ethoxyviburtinal of Valeriana jatamansi Jones on the expression of enterochromaffin cells and mast cells in rats with irritable bowel syndrome

作　　者：肖丛瑞 陶丝雨 闫兴丽[2] 高增平[1] 史瑞瑞[1] 王娟[1] 王晶[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]北京中医药大学基础医学院

出　　处：《北京中医药大学学报》2016年第1期21-25,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.81374044)

摘　　要：目的探讨蜘蛛香成分11-ethoxyviburtinal对肠易激综合征(IBS)大鼠肠嗜铬细胞(EC)及肥大细胞(MC)表达的影响。方法 SD雄性大鼠随机分为空白对照组、模型组、匹维溴铵(25 mg/kg)组和蜘蛛香成分11-ethoxyviburtinal大、中、小剂量(1.2、0.6、0.3 mg/kg)组。采用孤养复合慢性不可预见性应激法建立IBS大鼠模型,免疫组化法检测大鼠结肠5-羟色胺(5-HT)免疫反应阳性细胞EC数量的变化,甲苯胺蓝染色法检测大鼠结肠MC数量的变化。结果孤养复合慢性不可预见性应激可造成大鼠结肠5-HT免疫反应阳性细胞EC数量显著增加(P<0.01),结肠MC数量显著增多(P<0.05)。蜘蛛香成分11-ethoxyviburtinal大剂量可显著减少结肠5-HT免疫反应阳性细胞EC和MC数量(P<0.05)。结论蜘蛛香成分11-ethoxyviburtinal可抑制IBS大鼠结肠EC和MC的表达。Objective To investigate the effects of 11-ethoxyviburtinal of Valeriana jatamansi Jones on the expression of enterochromaffin cells（ EC） and mast cells（ MC） in rats with irritable bowel syndrome（ IBS）. Methods The IBS rat model was induced by chronic unpredictable stress with isolation. Male SD rats were randomly divided into six groups： control group,model group,pinaverium group at the dose of 25 mg/kg,and high-,mid- and low- dose of 11-ethoxyviburtinal group at dose of 1. 2,0. 6,and 0. 3mg/kg respectively. The number of 5-HT positive immunoreactive colonic EC was detected by the method of immunohistochemistry and that of colonic MC was measured with toluidine blue staining. Results Compared with control group,the number of 5-HT positive immunoreactive colonic EC and MC increased significantly in model group（ P 0. 05）. The high dose of 11-ethoxyviburtinal could significantly decrease the number of colonic EC and MC（ P 0. 05）. Conclusion The 11-ethoxyviburtinal of Valeriana jatamansi Jones could significantly reduce the number of 5-HT positive immunoreactive 5-HT positive immunoreactive EC and MC in colon tissue of rats with IBS.

关 键 词：蜘蛛香 11-ethoxyviburtinal 肠易激综合征 肠嗜铬细胞 肥大细胞 大鼠 

分 类 号：R285.5[医药卫生—中药学]