黄芪注射液对视神经牵拉伤大鼠NGF、TrkA表达的影响  

Astragalus Injection mediates the expressions of NGF and TrkA in retina tissue of rats optic nerve stretch injury model

作　　者：谷新怡 刘爱伟[1] 苏艳[2] 闫晓玲[2] 吴鲁华[3] 夏燕婷[2] 赵朋波 周剑[2] 

机构地区：[1]北京中医药大学,北京100029 [2]北京中医药大学东方医院 [3]北京中医药大学第三附属医院

出　　处：《北京中医药大学学报》2016年第1期26-29,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.81173307)

摘　　要：目的观察黄芪注射液对视神经牵拉伤大鼠视网膜组织神经生长因子(NGF)及酪氨酸激酶受体A(Trk A)蛋白表达的影响。方法将48只Wistar雄性大鼠随机分为假手术组、模型组及黄芪治疗组,每组16只大鼠。横向定量牵拉法制作大鼠视神经损伤模型,假手术组仅暴露视神经,不予牵拉;术后黄芪治疗组给予黄芪注射液腹腔注射,模型组给予生理盐水腹腔注射;治疗14 d后取大鼠视网膜组织,采用蛋白免疫印迹法(Western blot)检测NGF和Trk A蛋白表达变化。结果与假手术组比较,视神经牵拉伤模型大鼠视网膜组织NGF、Trk A蛋白表达显著下降(P<0.05);给药14 d后,与模型组比较,黄芪治疗组大鼠视网膜组织NGF及Trk A蛋白表达明显增加(P<0.05)。结论黄芪注射液能够上调大鼠视网膜组织NGF、Trk A的蛋白表达,具有视神经保护作用。Objective To observe the effects of Astragalus Injection（ AI） on the expressions of nerve growth factor（ NGF） and tyrosine kinase receptor A（ Trk A） in retinal tissue of rat model of optic nerve injury by stretch. Methods Altogether 48 healthy male Wistar rats were randomly divided into three groups： sham group,model group and AI group（ n = 16 each）. The rat model of optic nerve injury was induced by horizontal stretching the optic nerve of left eye by tensiometer with 0. 10 N for 20 seconds,and the rats of sham group were only exposed optic nerves,without undergoing stretching. After successful modeling,the rats of AI group were injected intraperitoneally（ i. p.） AI and the those of model group were i. p. normal saline for consecutive 14 days. The retinal tissues of all rats were dissected out of the eye balls and the expressions of NGF and Trk A were detected by Western Blot. Results Compared with the sham group,the expression levels of NGF and Trk A in the model group decreased significantly（ P 0. 05）. After 14-day-treatment,the expressions of NGF and Trk A of retinal tissues increased in AI group compared with the model group（ P 0. 05）. Conclusion The protection of AI against optic nerve injury was proved by upregulating of the expressions of NGF and Trk A in the rat retinal tissue.

关 键 词：视神经损伤 黄芪注射液 神经生长因子 酪氨酸激酶受体A 大鼠 

分 类 号：R285.5[医药卫生—中药学]