三七Y病毒部分ci基因和cp基因的克隆及序列分析  

Cloning and Sequence Analysis of Partial ci Gene and cp Gene of Panax Virus Y

作　　者：李梅蓉 李晓静[1] 包改丽[1] 朱静[1] 杨馨[1] 谭冠林[2] 兰平秀[3] 李凡[1] 

机构地区：[1]云南农业大学,农业生物多样性与病虫害控制教育部重点实验室,云南昆明650201 [2]云南农业大学教务处现代教育技术中心,云南昆明650201 [3]云南农业大学动物科学技术学院,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2016年第1期35-42,共8页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家自然科学基金(31160360);云南省高校科技创新团队支持计划(云教科[2014]22号)

摘　　要：对云南文山地区三七上获得的三七Y病毒（Panax virus Y,PnVY）不同分离物进行了部分ci基因和cp基因的克隆及序列分析,获得的部分ci基因长657 nt,编码219个氨基酸;获得的部分cp基因长834 nt,编码278个氨基酸。所获得的11个PnVY分离物的ci基因之间核苷酸和氨基酸的同源性分别为80.8%～99.8%和91.3%～100%,与其他PnVY分离物的同源性分别为80.8%～98.8%和92.7%～99.5%。所获得的18个PnVY分离物的cp基因核苷酸和氨基酸序列同源性分别为91.8%～99.9%和93.9%～100%,与其他PnVY分离物的同源性分别为92.0%～100%和95.7%～100%。系统进化树分析表明,基于ci基因可以将PnVY分为2个大组,第Ⅱ组分离物之间的差异相比第Ⅰ组的复杂;基于cp基因可以将PnVY分为3个大组,第Ⅲ组分离物之间差异最大。基于ci基因和cp基因的分析结果均表明,PnVY不同种群间存在较为丰富的遗传多样性,但尚不清楚PnVY的分子变异是否与PnVY引起的多种症状类型有关。Partial ci and cp genes of different Panax virus Y （PnVY） isolates from Wenshan in Yunnan Province were amplified, cloned and sequenced. The partial ci gene obtained in this study consists of 657 nt, encoding 219 amino acid, while the partial cp gene consists of 834 nt, encoding 278 amino acid. The results showed that the nucleotide （nt） and amino acid （aa） sequences of ci gene of 11 PnVY Yunnan isolates shared 80.8%～99.8% and 91.3%～100% identity with each other, and shared 80.8%～98.8% and 92.7%～99.5% identity with the recorded PnVY isolates. The nt and aa sequences of cp gene of 18 PnVY Yunnan isolates shared 91.8%～ 99.9% and 93.9%～100% identity with each other, and shared 92.0%～100% and 95.7%～100% identity with the PnVY isolates deposited in GenBank. The available PnVY isolates could be divided into two groups according to the phylogenetic tree based on the nucleotide sequences of partial ci gene, and the isolates in groupⅡshowed more diversity compared with those in groupⅠ. While the PnVY isolates could be divided into three groups base on the cp nt sequence, and the isolates of group Ⅲ showed the highest diversity. The results indicated that high genetic diversity exists among different PnVY populations, but whether the molecular variations on PnVY genome related to the different symptoms of the disease is still unknown.

关 键 词：三七Y病毒 ci基因 CP基因 序列分析 分子变异 

分 类 号：S435.672[农业科学—农业昆虫与害虫防治]