云南省十字花科蔬菜根肿病的实时荧光定量PCR检测  

Detection of Plasmodiophora brassicae with Real-time Quantitative PCR in Yunnan Province

作　　者：李淼[1] 周丽洪[1,2] 刘雅婷[3] 刘峰[1] 杨俊[1] 姬广海[1] 

机构地区：[1]云南农业大学,云南省农业生物多样性利用与保护重点实验室,云南昆明650201 [2]四川理工学院生物工程学院,四川自贡643000 [3]云南农业大学农学与生物技术学院,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2016年第1期43-48,共6页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家自然科学基金(3136002,3460458);云南省高校科技创新团队支持计划(云科教[2014]22号);云南省重点新产品计划(2014BB005)

摘　　要：根肿病是十字花科蔬菜严重的土传病害之一。为快速和准确的检测根肿病菌在土壤中的含量,本研究利用实时荧光定量PCR技术,结合传统的生物学测定方法对云南省十字花科蔬菜基地22份土壤样品进行了检测。结果表明:土壤孢子数量和病情指数呈线性相关,随着土壤中根肿病孢子数量增加,生物学测定的病情指数也随之增加;所检测的土壤中孢子密度最高为1×108.96g-1,病情指数为95.56%,最低为1×103.12g-1,相应的病情指数为3.33%。该方法可以快速检测和定量土壤中病原菌,为种植十字花科蔬菜土壤的检测预报提供了有效的技术手段。Clubroot of crucifereais vegetables is one of the serious soil-borne diseases. The quickly and quantify detection of Plasmodiophora brassicae of 22 cruciferous vegetables soil samples in Yunnan were tested by using real-time quantitative PCR technology and traditional method. The results showed that the highest concentration of clubroot spores was 1 × 10^8.96 g^-1 while the disease index of 95.56%;the lowest concentration of clubroot spores was 1 × 10^3.12 g^-1 while the disease index of 3.33%. The disease index was linearly correlated with clubroot spores number. The disease index and the number clubroot spores were increased or decreased at the same time. This method can quickly and quantify detect the P.brassicae in soil and provide an effective techniques for cruciferous vegetables soil detection.

关 键 词：根肿病 芸薹根肿菌 生物学测定 实时荧光定量PCR 

分 类 号：S436.341.19[农业科学—农业昆虫与害虫防治]