水稻半显性矮秆基因Si-dd1的表型分析和精细定位  

Phenotypical Analysis and Fine Mapping of a Semi-dominant Dwarfism Gene Si-dd1 in Rice

作　　者：崔永涛[1] 吴立文[1] 胡时开[1] 任德勇[1] 葛常伟 叶卫军[1] 董国军[1] 郭龙彪[1] 胡兴明[1] 

机构地区：[1]中国水稻研究所水稻生物学国家重点实验室,杭州310006

出　　处：《中国水稻科学》2016年第2期152-160,共9页Chinese Journal of Rice Science

基　　金：深圳市科技计划资助项目(JCYJ20140504111101999);国家自然科学基金资助项目(31271700;31461143014)

摘　　要：以粳稻品种日本晴在组织培养过程中分离出来的一个半显性矮秆突变体Si-dd1为研究对象,通过形态学分析,发现与野生型日本晴相比,矮秆Si-dd1(AA)和半矮秆Si-dd1(Aa)植株的株高降低。结实率下降,生育期延长,一次枝梗和二次枝梗增加。激素处理结果表明突变体Si-dd1(AA)与野生型对油菜素内酯反应基本相同,而在高浓度赤霉素处理下,突变体Si-dd1(AA)表现为一定程度上的钝感。Western blot对GID2表达量分析也确定这一结果。组织切片实验表明,突变体Si-dd1(AA)相对于野生型叶片主脉气孔变小,叶肉细胞增多,茎维管束数目增加。遗传分析结果表明该突变体基因受一对核基因控制。进一步利用分子标记将该基因定位在水稻第6染色体约244kb区间内,目前该区段并未发现已报道的矮秆相关基因。A semi-dominant dwarf rice mutant(termed as Si-dd1)was obtained from japonica rice variety Nipponbare by tissue culture mutagenesis.Morphological analysis showed that Si-dd1(AA)and Si-dd1(Aa)exhibited shorter plant height,decreased seed-setting rate,delayed heading,increased number of primary rachis branch and secondary rachis branch compared to the wild type(WT,Nipponbare).Physiological test results showed Si-dd1 had similar response to 24-Epibrassinosteroid to the WT,while it was insensitive to Gibberellin at 10-4μmol/L.Moreover,Western blot results also confirmed GID2 expression level in Si-dd1 differed greatly from WT.The cytological analysis showed that Si-dd1 had smaller stomas in the leaf midrib,increased mesophyll cells and stem vascular bundles compared to WT.Genetic analysis and map-based clone showed that the Si-dd1 is controlled by a single semi-dominant gene,and locates in a 244 kb region on chromosome 6.

关 键 词：水稻 半显性 矮秆 遗传分析 图位克隆 

分 类 号：S511[农业科学—作物学]