国槐槐角种胚细胞悬浮培养的动力学研究  

Kinetics of Sophora japonica embryo cells in a suspension culture system

作　　者：陈红贤 于笑笑[1] 王晨阳[1] 张敏[1] 刘忠华[1] 

机构地区：[1]北京林业大学生物科学与技术学院,北京100083

出　　处：《浙江农林大学学报》2016年第2期272-279,共8页Journal of Zhejiang A&F University

基　　金：国家自然科学基金资助项目(J1103516);北京林业大学理科生物学基地国家自然科学基金子项目(ZD008)

摘　　要：对国槐Sophora japonica槐角种胚细胞悬浮培养的动力学进行研究，在优化细胞接种量的基础上，分析测定培养周期内不同培养阶段的槐角细胞生长和培养基中碳源、氮源、磷源的消耗，以及细胞的鲜质量、干质量和黄酮产量的变化，从而了解细胞生长、营养消耗与次生代谢产物积累的基本规律，为建立结构化动力学模型奠定基础。结果表明：①初始接种量为40．000g·L^-1时，最利于细胞的培养；②细胞的培养周期约为27d，经过27d的培养，生物量和黄酮的产量达到最高，分别为9．733g·L^-1和53．566mg·L^-1，对细胞比生长速率和黄酮比合成速率进行分析，得出黄酮的积累和细胞的生长为部分生长偶联型，在6～12d呈负相关，12～33d呈正相关；⑧基质中的碳源、氮源、磷源都是在0～6d被迅速消耗，到12d时消耗量达到总量的80．00％以上。在氮源的吸收上，铵态氮（NH4^＋）先于硝态氮（N03^-）的吸收。碳源、氮源、磷源的消耗与细胞生长和产物的积累密切相关。Based on the established cell suspension culture system of Sophora japonica embryos, its kinetics was investigated. The suspension cell system of S. japonica embryos influenced by inoculum size was studied with analysis during cell culture of kinetic parameters, such as cell growth; consumption of C, N, and P; as well as changes in cell fresh weight, dry weight, and flavonoid production at various stages in the culture procedure.Results revealed that.(1) the preferable inoculum size for suspension cell proliferation was 40.000 g·L-1.(2)The cell suspension culture cycle lasted about 27 d with maximum biomass in dry weight reaching 9.733 g·L-1and flavonoid production attaining 53.566 mg·L-1. Flavonoid production was semi-associated with cell growth of the S. japonica embryo being negatively related from 6-12 d and positively related from 12-33 d.(3) From 0-6 d sugar, N, and phosphate were absorbed quickly; after 12 d they were more than 80% absorbed. Also, NH4+was utilized faster than NO3-. Thus, proliferation and flavonoid production of S. japonica embryo cells was closely related to the consumption of sugar, N, and phosphate. This research could provide references for the scale-up culture of S. japonica embryos cell by bioreactor.

关 键 词：植物学 国槐槐角种胚 悬浮细胞 黄酮 动力学 基质营养物质 

分 类 号：S718.3[农业科学—林学] Q942[生物学—植物学]