DNA结合分化抑制蛋白1和血管内皮生长因子的表达及其与肿瘤微血管生成的相关性研究  

Correlation of expressions of DNA binding differentiation inhibitory proteins-1 and vascular endothelial growth factor with its microvascular generation

作　　者：武雪亮[1] 王立坤[2] 薛军[1] 杨东东[3] 屈明[1] 郭飞[1] 杨瑞敏[2] 刘博[4] 

机构地区：[1]河北北方学院附属第一医院血管腺体外科,河北张家口075000 [2]河北北方学院附属第一医院超声医学科,河北张家口075000 [3]河北北方学院附属第一医院胃肠外科,河北张家口075000 [4]河北北方学院附属第一医院病理科,河北张家口075000

出　　处：《中国临床药理学杂志》2016年第5期430-432,436,共4页The Chinese Journal of Clinical Pharmacology

基　　金：河北省卫计委医学科学研究重点课题计划基金资助项目(20150058);河北北方学院校级重大基金资助项目(ZD201310);河北省张家口市科技局指令性计划基金资助项目(1311055D)

摘　　要：目的研究结直肠癌组织中DNA结合分化抑制蛋白1(Id-1)和血管内皮生长因子(VEGF)的表达及其与肿瘤微血管生成、临床病理指标的相关性。方法收集我院50例结直肠癌组织和30例正常结直肠组织,用免疫组化法检测Id-1和VEGF的表达及CD34标记的微血管密度(MVD)。结果 Id-1和VEGF在结直肠癌组织中阳性表达为72%,76%,显著高于癌旁正常组织的24.00%,22.00%。结直肠癌组织中MVD为16.15±2.19,明显高于正常组织4.37±2.21(P<0.01);Id-1、VEGF和MVD三者均与肿瘤浆膜浸润、TNM分期、CEA(+)、脉管浸润、淋巴结转移及肝转移相关(均P<0.01)。癌组织中,Id-1和VEGF阳性组表达MVD值显著高于阴性组(P<0.01);结直肠癌组织中,Id-1与VEGF表达呈明显正相关性。结论 Id-1、VEGF高表达能诱导肿瘤微血管的生成,与结直肠癌的浸润及血行转移有关。Objective To explore the expressions of DNA binding differentiation inhibitory proteins-1（ Id-1） and vascular endothelial growth factor（ VEGF） in colorectal carcinoma tissues and its correlation with microvascular generation. Methods The expression of Id-1,VEGF and microvessel density（ MVD） in colorectal adenocarcinoma tissues（ CAT,50 cases） and normal colorectal tissues（ NCT,50 cases） were examined by immunohislochemistry. Results The positive expression of Id-1 and VEGF were 72. 00%,and 76% in CAT,which was significant higher with24. 00%,and 22. 00% in NCT（ P〈0. 01）. The MVD value（ 16. 15 ±2. 19） in CAT were significantly higher than those（ 4. 37 ± 2. 21） in NCT,and there were significant difference（ P〈0. 01）. Expression of Id-1,VEGF and MVD value were correlated with the depth of tumor invasion,TNM stage,CEA（ ＋）,lymph node metastasis,vessel invasion,and liver metastasis（ P〈0. 01）. The MVD vlaue with Id-1 and VEGF positive expression was significantly higher than those with Id-1 and VEGF negative expression（ P〈0. 01）. The expression of Id-1 in CAT showed significantly positive correlation with that of VEGF. Conclusion The high expression of Id-1,and VEGF induce microvessel,and were significant concerned with the invasion and hematogenous metastasis of colorectal carcinoma.

关 键 词：结直肠癌 DNA结合分化抑制蛋白1 血管内皮生长因子 微血管密度 相关性 

分 类 号：R965.1[医药卫生—药理学] R362[医药卫生—药学]