愈骨胶囊对兔椎间盘退变模型基质金属蛋白酶的影响  

Effect of Healing bone capsule n on matrix metalloproteinase in rabbit model of disc degeneratio

作　　者：柳立新[1] 张毅[2] 颜瑞健[3] 

机构地区：[1]安徽医学高等专科学校,合肥230601 [2]安徽省药物研究所,合肥230022 [3]浙江省立同德医院,杭州310012

出　　处：《中国临床药理学杂志》2016年第5期440-442,共3页The Chinese Journal of Clinical Pharmacology

基　　金：浙江省医药卫生科技计划基金资助项目(2014KYB052)

摘　　要：目的研究基质金属蛋白酶(MMPs)在椎间盘退变中的作用及愈骨胶囊对兔椎间盘退变模型MMPs的影响。方法将成年新西兰白兔按随机数字法分为正常组、模型组、实验组,每组20只。用手术方法建立兔椎间盘退变模型。实验组给予0.15 g·kg^(-1)愈骨胶囊,正常组和模型组给予等量纯化水,连续给药4周。用免疫组化法和免疫印迹法检测兔腰椎间盘组织中MMP-3的表达,依据试剂盒说明检测血清中钙(Ca)和碱性磷酸酶(ALP)含量,双能X线吸收法(DXA)测定椎间盘组织中骨密度(BMD)和骨矿(BMC)含量。结果模型组,MMP-3表达显著高于对照组(P<0.05),而愈骨胶囊组,MMP-3表达显著低于模型组(P<0.05)。模型组,血清Ca和ALP、BMD和BMC含量显著低于对照组;而愈骨胶囊组,血清Ca和ALP、BMD和BCM含量显著高于模型组(P<0.05)。结论愈骨胶囊可降低MMP-3表达,促进钙盐形成,加速钙离子沉积,有效改善椎间盘退变。Objective To investigate the role of metalloproteinase matrix（ MMPs） in the intervertebral disc degeneration（ IDD） and the effect of the Healing bone capsule（ HBC） on the MMPs of rabbit intervertebral disc degeneration model. Methods The adult New Zealand white rabbits were randomly divided into normal group,model group and experimental group of 20. The rabbit model of disc degeneration was established using surgical methods. The experimental group was treated with0. 15 g·kg^-1HBC,the normal group and the model group were given equal volume of purified water,for 4 weeks. The MMP-3 expression of rabbit IDD was detected by immunohistochemical method and Western blot. The serum calcium（ CA） and alkaline phosphatase（ ALP） levels were determined according to the kit. The bone density（ BMD） and bone mineral（ BMC） in IDD tissues were measured by dual energy X-ray absorption method. Results The MMP-3 expression in model group was significantly higher than control group（ P〈0. 05）,while it in experimental group was significantly lower than in the model group（ P〈0. 05）. The levels of Ca and ALP in serum,BMD and BMC in model group were significantly lower than the control group（ P〈0. 05）,while that in experimental group were significantly higher than the modelgroup（ P〈0. 05）. Conclusion The HBC can reduce the expression of MMP-3,promote the formation of calcium salt,accelerate calcium deposition,improve the intervertebral disc degeneration.

关 键 词：椎间盘退变 基质金属蛋白酶 愈骨胶囊 相关机制 

分 类 号：R681.53[医药卫生—骨科学] R285.5[医药卫生—外科学]