荔枝核总黄酮及罗格列酮对大鼠肝星状细胞HSC-T6 PPAR-γ和CTGF表达的影响  

Effects of total flavonoids of litchi and rosiglitazone on expression of PPAR-γ and CTGF in HSC-T6 rat hepatic stellate cells

作　　者：刘瑞[1] 陈桂泓 徐伶俐[1] 罗伟生[1] 

机构地区：[1]桂林医学院附属医院消化内科,541004

出　　处：《实用医学杂志》2016年第3期344-347,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:81360530)

摘　　要：目的:研究中药荔枝核总黄酮(TFL)对大鼠肝星状细胞(HSC-T6)增殖的影响并以西药罗格列酮作对照,探讨TFL抗肝纤维化的相关作用机制。方法:MTT法检测TFL对HSC-T6增殖的影响;Real-time PCR检测TFL和罗格列酮对HSC-T6过氧化物酶体增殖物激活受体γ(PPAR-γ)和结缔组织生长因子(CTGF)mRNA表达的影响;Western blot检测TFL和罗格列酮对HSC-T6 CTGF蛋白表达的影响。结果:TFL作用48、72 h可以明显抑制HSC-T6增殖,且在一定范围内随时间延长及药物浓度增加,抑制作用更加明显;TFL和罗格列酮处理72 h后均能上调HSC-T6 PPAR-γ mRNA的表达,下调CTGF mRNA和蛋白的表达,TFL作用随浓度增加逐渐增强。结论:TFL能够抑制HSC-T6增殖从而拮抗肝纤维化,且在一定范围内有剂量时间依赖性,此种抑制作用可能是通过上调PPAR-γ,下调CTGF的表达来实现的。Objective To investigate effects of total flavonoids of litchi (TFL) on the proliferation of rat hepatic stellate cells (HSC-T6) in comparison with western medicine rosiglitazone, and to explore the mechanism of anti hepatic fibrosis of TFL. Methods Effect of TFL on proliferation of HSC-T6 was examined by MTT. The expression of peroxisome proliferator-activated receptor-γ (PPAR-γ) mRNA, connective tissue growth factor (CTGF) mRNA in HSC-T6 cells exposured were examined by real-time quantitative PCR. Effects on HSC-T6 CTGF protein from TFL and rosiglitazone were detected by Western bloting. Results The expression of PPAR-γ mRNA was upregulated and the expression of CTGF mRNA and protein was downregulated after exposure to TFL and rosiglitazone for 72 hours. And the effect of TFL increased with the increase of concentration. Conclusion TFL can inhibit the proliferation of HSC-T6 and antagonizing liver fibrosis. This mechanism may be associated with the upregulation of PPAR-γ expression and the downregulation of CTGF expression.

关 键 词：荔枝核总黄酮 罗格列酮 大鼠肝星状细胞 过氧化物酶体增殖物激活受体γ 结缔组织生长因子 

分 类 号：R285.5[医药卫生—中药学]