黄皮叶提取物对TNF-α分泌的影响及其机制  

Extract of HUANGPI inhibits secretion of TNF-α via TLR4/MyD88/TRAF6 pathway

作　　者：李娟[1] 刘天旭[1] 蒋国君[1] 黄桂红[1] 黄俊何[1] 陶丽群 朱钊铭 

机构地区：[1]桂林医学院第二附属医院,541199

出　　处：《实用医学杂志》2016年第3期367-370,共4页The Journal of Practical Medicine

基　　金：广西自然科学基金项目(编号:2012GXNSFAA053079);广西中医药科技专项项目(编号:GZKZ10-124);桂林市科学研究与技术开发计划项目(编号:20120121-1-17;20140120-1-9)

摘　　要：目的:探索黄皮叶提取物对TNF-α的影响及对TNF-α蛋白调控是否通过TLR4/My D88/TRAF6信号通路。方法:ELISA法检测TNF-α含量;MTT试验检测黄皮叶提取物和LPS对RAW264.7细胞抑制率的影响;对细胞随机分组,Western Blot法检测分组后TLR4、TRAF6蛋白表达。结果:黄皮叶提取物、LPS对细胞生长无明显抑制;黄皮叶提取物使TNF-α水平降低;与LPS组比较,LPS+My D88抑制剂组、LPS+黄皮叶提取物组、LPS+黄皮叶提取物+My D88抑制剂组明显抑制TNF-α分泌,差异有统计学意义(P<0.05),分别使TLR4和TRAF6蛋白降低至74%、21%,70%、27%,44%、8.5%。结论:黄皮叶提取物抑制TNF-α形成并主要介导TLR4/My D88/TRAF6信号通路中My D88依赖信号通路抑制TNF-α形成。Objective To investigate whetherthe extract of HUANGPI inhibitthe secretion of TNF-α via TLR4/MyD88/TRAF6 signaling pathway. Methods ELISA assay was performed to determine TNF-α level in cell culture medium. MTT assay was used to detect the effects of the extract of HUANGPI and LPS on the viabilities of RAW 264.7 cells. Proteinexpressions of TLR4 and TRAF6 were detected by Western blotting assay. Results The extract of HUANGPI inhibited the secretion of TNF-α in a dose-dependent manner. Compared to LPS group, were TNF-α was significantly suppressed in the cells in LPS＋MyD88 inhibitor group, LPS＋extract group and LPS ＋extract＋MyD88 inhibitor group, with the corresponding reductions of TLR4 and TRAF6 protein expression at74% and21%,70% and27%,44% and8.5%, respectively. Conclusion MYD88-dependent signaling pathway might be involved in the mechanism underlying the effect of the extract of HUANGPI on suppressing LPS-indueed inflammation.

关 键 词：黄皮叶提取物 炎症因子 信号通路 RAW264.7细胞 

分 类 号：R285[医药卫生—中药学]