CRISPR/Cas9技术在分子生物学创新性实验教学中的应用  

The application of CRISPR/Cas9 technology in the innovative experimental teaching of molecular biology

作　　者：谢胜松[1] 刘向东[2] 曾翠平[1] 马云龙[2] 王海燕[3] 

机构地区：[1]华中农业大学动物医学院动物生理生化教研室,武汉430070 [2]华中农业大学农业动物遗传育种与繁殖教育部重点实验室,武汉430070 [3]华中农业大学信息学院,武汉430070

出　　处：《生命的化学》2016年第1期117-125,共9页Chemistry of Life

基　　金：中央高校基本科研业务费专项资金项目(2662015BQ004;2662015BQ005);国家自然科学基金项目(31301226)

摘　　要：实验教学是高校分子生物学专业人才培养过程中不可或缺的关键环节,开发综合性和设计性实验项目可提高分子生物学实验教学的质量。CRISPR/Cas9是近年快速发展起来的基因组编辑技术,目前已成功应用于诸多物种,包括猪、兔和农作物等。以CRISPR/Cas9基因组编辑技术为核心,本实验室开设了一门名为"基因组定点编辑技术在动植物中的应用"创新性实验教学课程。本文详细阐述了实施该课程的教学目标、重点与难点、实验原理、实验步骤及结果等。实践表明,此实验教学项目可巧妙地将生物化学、分子细胞生物学、基因工程技术、生物信息学等多学科知识点有机整合。采用此策略,不仅可巩固与加强学生从分子生物学理论课所学的基础知识,同时有利于提高其综合实验设计能力与培养创新精神。该创新性实验课程内容难度适中,包含有验证性和探索性实验,可在开设分子生物学专业的高校推广与试行。Experimental teaching plays an important role in cultivating the molecular biology talents in higher education. To improve the quality of experimental teaching of molecular biology, the comprehensive design of experiments must be developed. CRISPR/Cas9 is a genome editing technology which developed rapidly and has been successfully applied in many organisms, including pig, sheep, rabbit and crop plants. Based on the CRISPR/Cas9 technology, an innovative experimental teaching course named ＂Targeted genome editing technology and its application in animals and plants＂ has been constructed. In this study, the teaching objective, focus and difficulty, experimental principles, experimental procedures and results of this course are detailed. In fact, the innovative experimental teaching course is able to flexible integrated knowledge of biochemistry, molecular and cellular biology, technique for gene engineering and bioinformatics and so on. It indicates that applying this approach is not only beneficial to consolidate and strengthen students＇ knowledge which learning from course of molecular biology on basic theory, but also help students improve their ability about comprehensive designing experiments and the cultivation of the society required innovation. The content of integrated curriculum is not difficult, containing replication and exploratory experiments, which can be widely applied in the other colleges and universities.

关 键 词：教学改革 CRISPR/Cas9技术 分子生物学 创新性实验教学 

分 类 号：Q7-4[生物学—分子生物学] G642[文化科学—高等教育学]