猪胆汁炮制对黄连中生物碱类成分溶出的影响  

Effect of Being Processed on Dissolution of Alkaloids in Coptidis Rhizoma with Pig's Bile

作　　者：王静[1] 吕佳[1] 袁子民[1] 陈悦[1] 

机构地区：[1]辽宁中医药大学,辽宁大连116600

出　　处：《中国实验方剂学杂志》2016年第5期5-8,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81303225)

摘　　要：目的:比较黄连、胆黄连及其水煎液中生物碱类成分的含量,研究猪胆汁炮制对黄连中生物碱类成分溶出效果的影响,探讨胆黄连寒性增强的炮制机制。方法:采用紫外分光光度法(UV)测定黄连、胆黄连及其水煎液中总生物碱含量,检测波长345 nm。采用高效液相色谱法(HPLC)测定黄连、胆黄连及其水煎液中盐酸药根碱、盐酸巴马汀、盐酸小檗碱的含量,流动相乙腈(A)-磷酸三乙胺水溶液(B)梯度洗脱(0~15 min,25%~30%A;15~20 min,30%~55%A),流速1.0 m L·min^(-1),检测波长345 nm。结果:黄连水煎液、胆黄连水煎液中总生物碱的转移率分别为71.17%和78.50%;黄连水煎液中盐酸药根碱、盐酸巴马汀、盐酸小檗碱的转移率分别为73.93%,51.59%,56.16%;胆黄连水煎液中三者的转移率分别为80.42%,64.21%,64.79%。结论:黄连经猪胆汁炮制后能够增加黄连中生物碱类成分在水煎液中的溶出,可达到寒性增强的炮制目的。Objective： To compare contents of alkaloids between Coptidis Rhizoma,bile processed products of Coptidis Rhizoma and their water decoctions,dissolution of alkaloids in Coptidis Rhizoma was determined after being processed with pig＇s bile.Aim of this study was to explore processing mechanism of cold property enhancement of bile processed products. Method： Total alkaloids in Coptidis Rhizoma,bile processed products and their water decoctions were determined by UV.Contents of berberine,jatrorrhizine and palmatine in Coptidis Rhizoma,bile processed products and their water decoctions were determined by HPLC,mobile phase was acetonitrile-phosphate triethylamine solution by gradient elution at room temperature,detection wavelength was set at 345 nm. Result： Transfer rates of total alkaloids from water decoctions of Coptidis Rhizoma and its bile processed products were 71.17% and 78.50%;transfer rates of jatrorrhizine,palmatine and berberine in water decoction of Coptidis Rhizoma were 73.93%,51.59% and 56.16%;transfer rates of jatrorrhizine,palmatine and berberine in water decoction of bile processed products of Coptidis Rhizoma were 80.42%,64.21% and 64.79%,respectively. Conclusion： Being processed with pig＇s bile can raise transfer rate of total alkaloids in water decoction of Coptidis Rhizoma,and then to achieve purpose of cold property enhancement of bile processed products of Coptidis Rhizoma.

关 键 词：黄连 胆黄连 生物碱 转移率 盐酸药根碱 盐酸巴马汀 盐酸小檗碱 

分 类 号：R283.1[医药卫生—中药学] R283.2[医药卫生—中医学]