紫金化毒栓治疗宫颈炎的体内外药效学评价  

Pharmacodynamics of Zijin Huadu Suppository in Treatment of Cervicitis

作　　者：郭姗姗[1] 高英杰[1] 包蕾[1] 金亚宏[1] 李艳英 崔晓兰[1] 萧伟[3] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]北京因科瑞斯药物研究院有限公司,北京102209 [3]江苏康缘药业股份有限公司,江苏连云港222001

出　　处：《中国实验方剂学杂志》2016年第5期115-122,共8页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国中医科学院中药研究所重点学科联合课题项目(2011ZDXK-03)

摘　　要：目的:观察紫金化毒栓在体内外对病毒、细菌、苯酚致宫颈炎的药效作用,为评价其治疗宫颈炎的药效作用提供实验依据。方法:SD大鼠70只,随机分为正常组,模型组,妇宁栓组(0.15 g·kg^(-1)·d^(-1)),聚甲酚磺醛栓组(8.4 mg·kg^(-1)·d^(-1))及紫金化毒栓高、中、低剂量组(1.2,0.6,0.3 g·kg^(-1)·d^(-1)),每组10只,除正常组外,其余各组采用针头刮擦3次,造成子宫表面损伤,每隔2日注入1次,共3次,造成感染模型,造模成功后阴道内给药,每日1次,连续12 d,采用HE染色病理切片观察大鼠宫颈炎病变,采用ELISA检测宫颈组织中病毒的表达。体外实验观察对单纯疱疹病毒2型(HSV-2)的抑制作用,采用RT-PCR和CPE法观察紫金化毒栓对病毒的抑制作用、采用比浊法观察抑菌作用。结果:在HPV16感染的大鼠宫颈炎模型上,紫金化毒栓1.2,0.6,0.3 g·kg^(-1)·d^(-1)剂量组对大鼠宫颈组织中HPV16表达具有明显的抑制作用,1.2,0.6 g·kg^(-1)·d^(-1)剂量组对宫颈组织病理改变具有明显的抑制作用;在大肠埃希菌、淋球菌和金黄色葡萄球菌混合感染的大鼠宫颈炎模型上,紫金化毒栓1.2,0.6 g·kg^(-1)·d^(-1)剂量组对大鼠宫颈的病理改变具有明显的抑制作用;在化学物质致大鼠宫颈炎的模型上,紫金化毒栓1.2 g·kg^(-1)·d^(-1)剂量组对大鼠阴道、宫颈病变具有明显的抑制作用。紫金化毒栓在体外对疱疹病毒2型、人乳头瘤病毒16型、金黄色葡萄球菌、白色葡萄球菌、表皮葡萄球菌、乙型链球菌、大肠埃希菌、绿脓杆菌、变形杆菌、淋球菌、白色念珠菌具有明显的抑制作用。结论:紫金化毒栓在体外对病毒、细菌均有明显的抑制作用,对苯酚致宫颈炎的具有明显的改善作用。Objective： To observe the pharmacodynamic effect of Zijin Huadu suppository on treatment of cervicitis induced by virus, bacteria and phenol in vivo and in vitro, and provide experimental basis for evaluating its therapeutic effects for cervicitis. Method： The 70 SD rats were randomly divided into normal group, model group, Funing suppository group （0.15 g · kg^-1·d^-1,policresulen suppository group （8.4 mg · kg^-1·d^-1, Zijin Huadu suppository high dose group, middle dose group and low dose group （1.2, 0.6, 0.3 g · kg^-1·d^-1, n=10 in each group. Needle scraping was done for three times in all the other groups except the normal group to induce surface damage of uterus, and infusion was done once every 2 days for 3 times in total to induce infection models. After successful modeling, vagina administering was done once a day for continuous 12 days. HE staining pathological dyeing was used to observe the cervicitis lesions of rats;ELISA assay was used to detect the expression of virus in cervical tissues;in vitro test was used to observe its inhibitory effect on herpes simplex virus-2 （HSV-2）; RT-PCR and CPE method were used to observe the inhibitory effect of Zijin Huadu suppository on virus replication and its bactriostasis activity was observed by turbidimetry. Result： The results demonstrated that in HPV16-infected cervicitis rat models, Zijin Huadu suppositories at doses of 1.2, 0.6, 0.3 g · kg^-1·d^-1 ad significant inhibitory effect on expression of HPV16 in cervical tissues, and the doses of 1.2, 0.6 g · kg^-1·d^-1 had significant inhibitory effect on pathological changes of cervical tissues. In cervicitis rat models with mixed infection of Escherichia coli, Neisseria gonorrhoeae and Staphylococcus aureus, Zijin Huadu suppositories at doses of 1.2, 0.6 g · kg^-1·d^-1 had significant inhibitory effect on pathological changes of cervical tissues. In cervicitis rat models induced by chemical substances, Zijin Huadu suppositories at dose of 1.2 g · kg^-1·d^-1 had significant inhibi

关 键 词：紫金化毒栓 宫颈炎 人乳头瘤病毒16型 细菌混合感染 苯酚胶浆 

分 类 号：R285.5[医药卫生—中药学]