二仙汤对去卵巢大鼠心脏电活动的影响  

Electrophysiological Effects of Erxian Decoction on Ovariectomized Rat Heart

作　　者：赵兰平[1] 陈彦静[2] 薛淑芳[1] 向丽华[2] 王震[2] 焦宏[1] 张治国[2] 鞠大宏[2] 

机构地区：[1]河北北方学院,河北张家口075000 [2]中国中医科学院中医基础理论研究所,北京100700

出　　处：《中国实验方剂学杂志》2016年第5期136-140,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中国中医科学院自主选题研究项目(YZ-1318);河北省卫生厅医学科学研究重点项目(20130038)

摘　　要：目的:探讨二仙汤对去卵巢大鼠心脏的电生理效应。方法:选用健康13周龄雌性SPF级SD大鼠32只,随机分为4组,分别为假手术组、模型组、雌激素组、二仙汤组,每组8只。大鼠分组后开始灌胃给药,1次/d,连续12周。二仙汤组ig7.5 g·kg^(-1)·d^(-1);雌激素(戊酸雌二醇)组ig 0.2 mg·kg^(-1)·d^(-1);假手术组和模型组灌服等体积纯净水。大鼠麻醉后,以针刺电极记录标准Ⅱ导联ECG,随后玻璃微电极细胞内电位记录技术记录离体心室流出道标本自发节律和心室肌动作电位,并分析各项参数的变化。结果:与假手术组比较,模型组大鼠P波,R波和T波振幅明显减小,P波时间,PR间期,QT间期,T波时间明显缩短(P<0.05,P<0.01),易出现T波低平或倒置,二仙汤组和雌激素组均可一定程度的逆转上述ECG改变。模型组大鼠离体心室流出道自发节律明显升高,更易触发心律失常,二仙汤组和雌激素组自发节律明显减慢,并可使去甲肾上腺素(NE)诱发的自发节律异常明显改善。模型组大鼠心室肌动作电位时程(APD)明显缩短,以APD50缩短更明显,二仙汤可使APD50的缩短明显改善,均具有统计学差异(P<0.05,P<0.01)。结论:二仙汤可明显改善去卵巢大鼠心脏电活动的改变,对由于雌激素水平下降出现的心律失常有一定的治疗作用。Objective： To explore the electrophysiological effects of Erxian decoction on ovariextomized rat heart. Method： Thirty-two female healthy rats were randomly divided into four groups：sham operated control group, ovariectomized group, estrogen group, and Erxian decoction group. The rats in estrogen group and Erxian decoction group were intragastrically administrated with estradiol valerate （0.2 mg · kg^-1·d^-1） and Erxian decoction （7.5 g · kg^-1·d^-1） respectively, ig, for 12 weeks. The rats in sham operated control group and ovariectomized group were intragastrically administrated of with equivalent volume of purified water for 12 weeks. The Ⅱ lead ECGs of anesthetic rats were recorded. And the spontaneous activities of left ventricular outflow tract and the fast action potentials （APs） of left ventricular muscle were also recorded by intracellular electrode. Result： Compared with sham operated control group, P, R and T amplitude decreased notably, and P duration, PR interval, QT interval, and T duration decreased significantly in ovariectomized group （P〈0.05, P〈0.01）. In estrogen group, T amplitude, P duration, PR interval and QT interval were reversed to control group. In Erxian decoction group, R amplitude, T amplitude, P duration and PR interval were reversed to control group. Compared with control group, the pacemaker rates of ventricular outflow tract and norepinephrine（NE） induced abnormal pacemaker activities increased significantly in ovariectomized group. In estrogen and Erxian decoction groups, the pacemaker activities of ventricular outflow tract decreased were markedly compared with lower than ovariectomized group, and NE-induced spontaneous arrhythmia was significantly relieved. That is, estrogen and er-xian decoction depressed the activity of latent pacemaker cell in ventricular outflow tract and had less possibility to lead to arrhythmia. Compared with control group, action potential duration （APD） was shortened significantly in ovariectomized group, espec

关 键 词：二仙汤 去卵巢大鼠 心电图 心室流出道 动作电位 

分 类 号：R285.5[医药卫生—中药学]