双合汤对酒精性股骨头坏死的干预作用  

Intervention Effects of Shuanghe Tang on Alcohol-induced Psteonecrosis of Femoral Head

作　　者：刘华[1] 魏爱淳 秦广珍[1] 管晓军[1] 包海林 

机构地区：[1]海安县中医院,江苏海安226600

出　　处：《中国实验方剂学杂志》2016年第5期141-146,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏省南通市市级科技计划项目(HS13933)

摘　　要：目的:探讨双合汤对酒精性股骨头坏死(AOFH)的干预作用。方法:将60只中国白兔随机分成正常组,模型组,美多巴组(0.125 g·kg^(-1)),双合汤低、中、高剂量组(9,18,27 g·kg^(-1))。除正常组外,各组给予中国白酒(乙醇含量56%)10m L·kg^(-1)·d^(-1)ig,在造模的同时,各给药组均ig给药,正常组、模型组给予生理盐水,预防性ig给药8周后取血测定血液流变学指标及血清总胆固醇(TC),甘油三酯(TG),高密度脂蛋白(HDL),低密度脂蛋白(LDL),骨钙素(BGP),血管内皮生长因子(VEGF),骨形态发生蛋白-2(BMP-2)的水平。采血后,处死实验动物,观察组织病理学的变化。采用RT-PCR技术检测股骨头组织中过氧化物酶体增殖子活化受体γ(PPARγ)mRNA与成骨基因骨钙素(OC)mRNA的表达。结果:与正常组比较,模型组中TC,TG,LDL的水平及血液流变学指标均显著升高(P<0.01),HDL,BGP,VEGF,BMP-2的水平均显著下降(P<0.01)。与模型组比较,双合汤中、高剂量组可显著降低TC,TG,LDL的水平及血液流变学指标与升高HDL,BGP,VEGF,BMP-2的水平(P<0.01)。模型组中空骨陷窝率、最大脂肪细胞平均直径均显著高于正常组(P<0.01),成骨细胞计数显著低于正常组(P<0.01)。双合汤中、高剂量组空骨陷窝率、最大脂肪细胞平均直径均明显低于模型组(P<0.01),成骨细胞计数均明显高于模型组(P<0.01)。模型组中PPARγmRNA表达值显著高于正常组(P<0.01),OC mRNA表达值显著低于正常组(P<0.01)。双合汤中剂量组中PPARγmRNA表达值明显低于模型组(P<0.01),OC mRNA表达值明显高于模型组(P<0.01)。结论:使用双合汤干预能有效改善酒精引起的高脂血症,改善血液流变学,降低血液黏度,有效抑制酒精诱导的兔股骨头内骨髓基质细胞的成脂分化、维持其成骨分化,从而防治AOFH。Objective： To discuss the interventional effect of Shuanghe Tang on alcohol-induced osteonecrosis of the femoral head （AOFH）. Method： Totally 60 Chinese white rabbits were randomly divided into the normal group, the model group, the madopar group, and low, medium and high-dose Shuanghe Tang groups. Except for the normal group, all of the remaining groups were lavaged with Chinese liquor （alcohol content 56%） 10 mL · kg^-1·d^-1 Meanwhile, each drug-treated group was lavaged with drugs. The normal group and the model group were given normal saline. After 8 weeks of reventive administration, the rabbits were sacrificed to collect blood for determination of the index of hemorheology, serum total cholesterol （TC）, triglyceride （TG）, high density lipoprotein （HDL）, low density lipoprotein （LDL）, bone glaprotein （BGP）, vascular endothelial growth factor （VEGF） and bone morphogenetic protein-2 （BMP-2）. The rabbits were killed after blood collection, and histopathological changes were detected. The expression levels of PPARγ mRNA and OC mRNA in femoral heads were analyzed by RT-PCR. Result： Compared with the normal group, the level of TC, TG, LDL and the index of hemorheology in the model group were significantly increased（P〈0.01）, and the levels of HDL, BGP, VEGF and BMP-2 were significantly decreased （P〈0.01）. Compared with the model group, the level of TC, TG, LDL and the index of hemorheology were significantly decreased in the medium and high-dose Shuanghe Tang groups, and the level of HDL, BGP, VEGF,BMP-2 and Osteoblast count were significantly increased （P〈0.01）. Compared with the normal group, the empty bone lacuna rate and average diameter of the max adipocyte in the model group were significantly increased（P〈0.01）,and the osteoblasts count was significantly decreased （P〈0.01）. Compared with the model group, the empty bone lacuna rate and average diameter of the max adipocyte were significantly decreased in the medium and high-dose Shuangh

关 键 词：酒精性股骨头坏死 双合汤 脂质 血黏度 基因 

分 类 号：R285.5[医药卫生—中药学]