利用知识发现工具Arrowsmith探讨天佛参口服液及其有效成分与结肠癌的相关性  

Investigate Tianfoshen Oral Liquid and Correlation Between Its Effective Components and Colorectal Cancer Using Knowledge-Discovery Tool Arrowsmith

作　　者：孙丽华[1] 汪思亮[1] 曹玉珠[1] 陈文星[1,2,3] 王爱云[1,2,3] 祝娉婷[1,2,3] 王欢 王恒斌 陆茵[1,2,3] 

机构地区：[1]南京中医药大学药学院,南京210023 [2]江苏省药效与安全性评价重点实验室,南京210023 [3]南京中医药大学江苏省中医药防治肿瘤协同创新中心,南京210023 [4]常熟雷允上制药有限公司,江苏常熟215500

出　　处：《中国实验方剂学杂志》2016年第5期215-220,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏省科技厅项目(BY2015008-02);2013年江苏高校优秀科技创新团队计划项目;江苏省研究生科技创新计划项目(KYZZ_0270;KYZZ15_0271)

摘　　要：目的:探讨天佛参口服液治疗结肠癌的可能机制。方法:运用非相关文献知识工具Arrowsmith软件。结果:Arrowsmith软件的初步探索结果显示天佛参口服液中的有效成分对Toll样受体(toll-like receptors,TLRs),血管内皮生长因子(vascular endothelial growth factor,VEGF),环氧合酶-2(cyclooxygenase-2,COX-2)和凋亡相关因子可能具有一定的调节作用,同时上述指标的异常表达也是造成结肠癌的重要相关遗传因素。结论:天佛参口服液在体内外均有显著抑制结肠癌的效应,其机制可能是通过下调结肠癌组织中Toll样受体4(toll-like receptor 4,TLR4)与COX-2的表达、降低组织中异常高表达的VEGF,促进促凋亡基因的表达与活力等环节发挥治疗结肠癌的作用,能为实验室进一步开展机制验证性研究提供有力的参考。Objective： To explore the potential mechanism of Tianfoshen oral liquid in the treatment of colorectal cancer. Method： Software of Arrowsmith based on the theory of Swan＇s noninterative literature-bases knowledge discovery was applied. Result： The preliminary investigation results by Arrowsmith software showed that the effective components of Tianfishen oral liquid might havecertain ability to regulate the expressions of toll-like receptors （TLRs）, vascular endothelial growth factor （VEGF）,cyclooxygenase-2 （COX-2）, and apoptosis related factors, and the abnormal expressions of the above indicators were also the key genetic factors for colorectal cancer. Conclusion： Tianfishen oral liquid has significant effect on inhibiting colorectal cancer in vitro and in vivo, and the mechanism may be associated with down-regulating the expressions of toll-like receptor 4 （TLR4） and COX-2 in colorectal cancer tissues, reducing abnormally high expression of VEGF in tissues, and promoting expression and vitality of apoptosis genes. This can provide strong reference for the laboratory to conduct further mechanism verification studies.

关 键 词：天佛参口服液 有效成分 结肠癌 Arrowsmith 

分 类 号：R287[医药卫生—中药学]