上海市重症急性呼吸道感染儿童中可检出多种型别的人腺病毒  

Prevalence of multiple types of human adenovirus infection among children with severe acute respiratory infection in Shanghai

作　　者：李亚敏[1] 刘高山[1] 周为民[1] 赵彦杰[1] 李玉川[2] 沈军[2] 谭文杰[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所应急技术中心,北京102206 [2]复旦大学附属儿童医院,上海201102

出　　处：《中华实验和临床病毒学杂志》2016年第1期10-13,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的了解上海市重症急性呼吸道感染（SARI）儿童中人腺病毒（HAdV）的感染情况及其分型特征。方法2013年6月至2014年3月从上海复旦大学附属儿科医院收集441份重症急性呼吸道感染患儿鼻咽抽吸物（NPAs）。采用巢式PCR的方法进行人腺病毒的检测，对阳性样本进行分型分析及基本流行病学特征分析。结果人腺病毒共检出51例（11．6％，95％CI8．6％～14．6％）阳性，以0．5—1岁之间的患儿感染率较高，分型分析发现感染者中B组所占比例最高，共33例（64．7％），其中HAdV-3共17例（33．3％），HAdV-7共检出16例（31．4％）；其次为C组共14例（27．5％）；HAdV—D（HAdV-8）与HAdV—E（HAdV-4）各1例；HAdV—F（HAdV-41）检出2例。结论人腺病毒在上海市重症急性呼吸道感染患儿中有较高的感染率．存在多种基因型且以HAdV—B中的HAdV．3、7型感染最为常见。Objeetive To investigate the typing profiles of human adenovirus infection among children with severe acute respiratory infection （SARI） in Shanghai. Methods A total of 441 nasopharyngeal aspirates （NPAs） were collected from hospitalized children with SARI in Shanghai Pediatrics Hospital Affiliated Fudan University from June 2013 to March 2014. Human adenovirus was detected by nested PCR followed molecular typing by sequencing. Then the phylogenetic and epidemiological analysis was conducted. Results From 441 hospitalized children with SARI, 51 of samples （11.6%, 95% CI： 8.6% - 14. 6% ） were detected as HAdV infection. Most of the SARI children with HAdV infection were distributed between 0. 5 to 5 years old. Typing data shown that HAdV-B （ 17 of HAdV-3, 16 of HAdV-7 ） was most dominant as 64. 7% （33/51） ,followed by HAdV-C as 27. 5% （ 14/51 ） ; either HAdV-D （ HAdV- 8） or HAdV-E （HAdV-4） contained only one case, and HAdV-F （HAdV-41） found in two cases. Conclusion Multiple species（B-F） of HAdVs were circulated among children with SARI in Shanghai, and HAdV-B （HAdV-3, HAdV-7） was the most predominant species of circulation.

关 键 词：重症急性呼吸道感染 儿童 人腺病毒 分型检测 

分 类 号：R725.6[医药卫生—儿科]