单纯疱疹病毒性角膜炎兔角膜表面温度的改变与角膜损伤关系的研究  

The experimental research of cornea superficial temperature in herpes simplex keratitis and its relationship with cornea lesion

作　　者：俞莹[1] 缪晚虹[1] 赵芳[1] 李菲菲[1] 

机构地区：[1]上海中医药大学附属曙光医院,201203

出　　处：《中华实验和临床病毒学杂志》2016年第1期19-22,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：上海市科委自然基金青年基金（12ZR1450600）;上海市卫计委三年行动计划（ZY3.JSFC-1-1019）

摘　　要：目的研究单纯疱疹病毒性角膜炎兔角膜表面温度的变化及其与角膜损伤的关系。方法采用HSV．1KOS病毒株感染兔角膜建立单纯疱疹病毒性角膜炎模型12只，对照组10只行单纯角膜上皮划开，不接种病毒。红外线测温仪检测角膜温度。造模后第l天、第3天、第7天、第14天观察角膜上皮病变面积、角膜基质混浊程度、新生血管生长情况并评分。结果测得单纯疱疹病毒性角膜炎兔角膜温度为31．14,4-1．12℃。造模后第1天、第3天、第7天、第14天角膜温度皆高于对照组（P〈0．01，P〈0．05）。第3天、第7天、第14天角膜基质明显混浊，且第3天、第7天混浊程度与角膜表面温度具有相关性（P〈0．05）。第14天角膜新生血管明显增多，与角膜表面温度具有相关性（P〈0．05）。结论单纯疱疹病毒性角膜炎角膜表面温度增高，且与角膜混浊程度、新生血管等角膜损伤表现有相关性。Objective To study the change of cornea superficial temperature of herpes simplex keratitis（HSK） rabbits and the relationship between corneal temperature with cornea lesion. Methods The right eyes of the 12 rabbits that were diagnosed as HSK by being inoculated HSV-1KOS virus to the cornea were brought into the experiment group. The 12 right eyes of the control group were dealt with incision on the cornea epithelial layer without virus. The corneal temperature was measured with the infrared thermometer. The scale of epithelia damage, lesion of stroma and neovascular were observed before and at the 1^st day, 3^rd day, 7^th day, 14^th day after establishment of the HSK model. Results The cornea superficial temperature of herpes simplex keratitis was 31.14 ± 1.12℃ The temperatures at the 3^rd day, 7^tb day, 14^th day were all higher than those of the control group （P 〈0. 01, P 〈0. 05）. The stroma opacity were rather serious at the 34 day, 7th day, 14th day, and the temperatures of the 3^rd day and 7^th day were relevant to the degrees of strnma opacity（P 〈0. 05）. The neovascular was significantly increased at the 14＇h day, which was relevant to the cornea temperature （P 〈 0. 05）. Conclusion The cornea superficial temperature was increased in the case of herpes simplex keratitis, and the temperature was relevant to the degree of stroma opacity and neovascular.

关 键 词：单纯疱疹病毒性角膜炎 角膜温度 角膜损伤 实验研究 

分 类 号：R752.11[医药卫生—皮肤病学与性病学] R772.21[医药卫生—临床医学]