尖锐湿疣患者皮损中TLR7／9相关信号通路蛋白的表达  

The expression of TLR7/9 and associated signaling pathway proteins in condyloma accuminatum lesions

作　　者：朱小霞[1] 周强[2] 程浩[2] 韩睿[2] 朱红明[1] 

机构地区：[1]宁波市第一医院,315010 [2]浙江大学医学院附属邵逸夫医院,杭州310016

出　　处：《中华实验和临床病毒学杂志》2016年第1期26-29,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：宁波市自然科学基金（2012A610218）;宁波市卫生局科研项目（2011804）;国家自然科学基金（81301375）

摘　　要：目的探讨TLR7／9及其信号通路在尖锐湿疣（condylomaacuminatum，CA）免疫发病机制中的可能作用。方法采用免疫组织化学sP法检测23例CA患者皮损TLR7／TLR9、IRF7和IRAK1的表达和分布情况，以13例边缘带正常皮肤的CA患者为对照，分别比较分析23例CA皮损与其中13例CA皮损边缘正常皮肤，以及13例CA皮损与其边缘正常皮肤中TLR7／TLR9、IRF7和IRAK1表达密度和阳性率的差异。结果TLR9在表皮角质形成细胞和真皮乳头层部分单核样细胞胞浆内表达；TLR7、IRF7、IRAK1表达阳性细胞主要位于真皮乳头层，均为胞浆表达。尖锐湿疣患者皮损真皮乳头层TLR9表达阳性细胞密度和阳性率均比边缘正常皮肤明显增高（P〈0．05）；TLR7、IRF7、IRAK1表达阳性细胞密度和阳性率与边缘正常皮肤无显著性差异（P〉0．05）。结论尖锐湿疣患者皮损中TLR9的表达水平上调，而TLR7、IRF7、IRAK1的表达水平未见明显改变。推测：尽管尖锐湿疣皮损TLR9的表达上调，但TLR7／9下游通路未被有效激活。故而未能激活抗HPV免疫反应，进而HPV病毒清除困难，并可能与临床上尖锐湿疣的易复发或迁延不愈有关。Objective To detect the expressions of TLR7/9, IRF7 and IRAK1 in condyloma accuminatum （CA） lesions and to investigate their possible roles in pathogenesis of CA. Methods The expressions of TLR7/9, IRF7 and IRAK1 in 23 cases of CA lesions were tested by immunohistochemistry. Results TLR9-positive cells were observed both in the epidermis and in the papillary layer of dermis. The density and positive rate of TLR9-positive cells in the papillary layer of CA lesions were significantly higher than those in their normal borderline tissues （P 〈 0. 05 ）. TLR7-positive cells, IRFT-positive ceils and IRAKl-positive cells were observed mostly in the papillary layer of dermis. There were no obvious difference of their density and positive rate between the CA lesions and their normal borderline tissues （ P 〉 0. 05 ）. Conclusions The TLR9 expression was up-regulated, while the TLR7, IRF7 and IRAK1 expression were un-changed in CA lesions. Our results suggested that the downstream of TLR7/9 signaling pathways in CA could not be provoked despite the up-regulation of TLR9 expression, which lead to the difficulty to HPV removal and the recurrence of CA.

关 键 词：尖锐湿疣 TLR7 TLR9 IRF7 IRAK1 乳头状瘤病毒 人 

分 类 号：R752.53[医药卫生—皮肤病学与性病学]