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机构地区:[1]宁波市中心血站,315000 [2]宁波市第二医院输血科,315000 [3]浙江医学高等专科学校,杭州310053
出 处:《中华实验和临床病毒学杂志》2016年第1期64-66,共3页Chinese Journal of Experimental and Clinical Virology
基 金:浙江省医学重点学科建设计划项目
摘 要:目的探讨丙型肝炎病毒核心抗原(HCV-cAg)酶联免疫检测(ELISA)在丙型肝炎早期诊断中的应用价值。方法83例抗.HCV阳性、500例ALT异常但抗-HCV阴性血清同时进行HCV—cAgELISA和HCVRT—PCR。结果83例抗-HCV阳性血清中HCV—cAg和HCVRNA阳性分别为33例和36例,其中32例共阳性;500例ALT异常但抗。HCV阴性的血清中有3例HCV—cAg和HCVRNA同为阳性。抗-HCV阳性和阴性血清HCV-cAg和HCVRNA检测结果符合率分别为94.0%和100%。结论HCV-cAgELISA敏感性与HCVRT—PCR类似,能够有效缩短窗口期,操作简便,费用低廉,在丙型肝炎早期诊断中具有很好的前景。Objective To evaluate the clinically applied valve of HCV core antigen enzyme-linked immunosorbent assay ( HCV-cAg ELISA) for early diagnosis of hepatitis C. Methods Both HCV RNA and HCV-cAg ELISA were used to detect 83 cases of anti-HCV positive serum samples and 500 cases of anti- HCV negative but ALT abnormal serum samples. Results In the 83 cases of anti-HCV positive serum samples, 33 cases were detectable for HCV-cAg while 36 cases were detectable for HCV RNA, in which 32 cases were double positive for both HCV-cAg and HCV-RNA. In the 500 cases of anti-HCV negative but ALT abnormal serum sample, only 3 cases were detectable for both HCV-cAg and HCV RNA. The coincidence rates of the two methods for detecting the anti-HCV positive serum samples and anti-HCV negative but ALT abnormal serum samples were 94. 0% and t00%, respectively. Conclusion The sensitivity and specificity of HCV-cAg ELISA is similar to that of HCV RT-PCR. The HCV-cAg ELISA has many advantages such as window-phase, decurtation, convenience and lower cost, which enable the assay a good clinically applied prospects for early diagnosis of hepatitis B.
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