脯氨酸和可溶性糖在南极冰藻低温适应机制中的作用  

Roles of Proline and Soluble Sugar in the Cold-adaptation of Antarctic Ice Microalgae

作　　者：王以斌[1,2] 缪锦来[1,2] 姜英辉[1,2] 刘芳明[1,2] 郑洲[1,2] 李光友[1,2] 

机构地区：[1]国家海洋局第一海洋研究所,青岛266061 [2]国家海洋局海洋生物活性物质重点实验室,青岛266061

出　　处：《生物技术通报》2016年第2期198-202,共5页Biotechnology Bulletin

基　　金：国家海洋局第一海洋研究所基本科研业务费专项(2015G10;2013G33);国家自然科学基金青年基金项目(31200097;31200272);海洋公益性行业科研专项(201305027;201405015)

摘　　要：对低温胁迫下两种南极冰藻胞内的脯氨酸和可溶性糖含量的变化进行分析研究,探讨两种冰藻对低温的适应性机制。测定不同培养温度和培养时间下两种南极绿藻细胞中的脯氨酸和可溶性糖含量,获得其在低温下随温度和胁迫时间变化的趋势,分析其产生变化的原因。结果显示,温度越低,塔胞藻Pyramidomonas sp.L-1胞内的脯氨酸和可溶性糖含量增加越明显。-5℃,培养48 h后,脯氨酸含量是初始值的6.5倍,可溶性糖含量增加60%。衣藻Chlamydomonas sp.L-4胞内脯氨酸的含量同样为温度越低,胞内脯氨酸的含量越高;但随时间变化趋势与塔胞藻Pyramidomonas sp.L-1相反,含量随温度作用时间的增加出现减少的趋势。-5℃,48 h后脯氨酸含量基本维持不变,可溶性糖含量增幅达90%。结果表明,脯氨酸和可溶性糖在南极冰藻的低温适应性机制中具有重要的作用,但在不同藻种中的作用机制不同。The content changes of proline and soluble sugar in two Antarctic ice microalgae under low temperature stress were measured and analyzed for understanding their cold-adaptation mechanisms. Measuring the proline and soluble sugar in two microalgae at different low temperatures and culturing time, the changing trend of them with the temperature and stress time at low temperature was obtained, and the reasons of it was analyzed. The lower the temperature was, the more significantly the contents of proline and soluble sugar in Pyramidomonassp. L-1 increased. The amount of proline was 6.5 times of initial one and the soluble sugar increased 60% after 48 h at-5℃. The content of proline in Chlamydomonas sp. L-4 was in the same way as Pyramidomonas sp. L-1, i.e., the lower the temperature was, the higher the content of proline was. However, the changing trend of the content with the time was in contrast, decreasing with the culturing time. The proline was almost unchanged after 48 hours at-5℃, while soluble sugar increased 1.9 times. In conclusion, proline and soluble sugar play an important role in the cold-adaptation mechanism of Antarctic ice microalgae, however, the action mechanism is different in the different algae species.

关 键 词：南极冰藻 低温适应性 脯氨酸 可溶性糖 

分 类 号：Q945[生物学—植物学]