羧胺三唑对大鼠佐剂性关节炎的治疗作用  

Therapeutic Effect of Carboxyamidotriazole on Adjuvant Arthritis in Rats

作　　者：朱蕾[1] 李娟[1] 郭磊[1] 于晓丽[1] 武丹威[1] 陈玮[1] 陈晨[1] 杜晓婉 张德昌[1] 叶菜英[1] 

机构地区：[1]中国医学科学院北京协和医学院基础医学研究所药理系,北京100005

出　　处：《中国医学科学院学报》2016年第1期49-54,共6页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金(81102454;81201728;81402943);"重大新药创制"科技重大专项(2014ZX09507003-003)~~

摘　　要：目的研究羧胺三唑(CAI)对大鼠佐剂性关节炎(AA)的治疗作用。方法大鼠随机分为正常对照组、溶剂对照组(聚乙二醇400组和生理盐水组)、CAI 3个剂量(10、20、40 mg/kg)组和阳性药地塞米松(Dex)组。采用弗氏完全佐剂诱导大鼠AA模型,进行关节炎评分、后肢X线摄片和组织病理学检查,检测大鼠炎症足爪组织中肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β和IL-6水平。结果 CAI能明显降低AA大鼠的关节炎指数,促进其体重的恢复,改善AA大鼠后肢关节放射学和组织病理学损伤(P<0.05或P<0.01)。CAI低、中、高剂量组和Dex组可显著降低TNF-α和IL-1β水平,CAI中、高剂量和Dex可显著降低IL-6水平(P<0.05或P<0.01)。结论 CAI对AA大鼠具有明显的治疗作用,其作用机制可能与其降低炎症部位的促炎细胞因子有关。Objective To study the effect of carboxyamidotriazole（ CAI） on adjuvant arthritis（ AA） in rats. Methods The rats were randomly divided into normal group,two vehicle groups（ polyethylene glycol 400 control and normal sodium control group）,CAI-treated groups（ 10,20,and 40 mg / kg） and positive control dexamethasone group. Freund＇s completed adjuvant was used to induce AA in rats. The arthritis index（ AI） was scored,and X-ray check of the hind limbs and histopathological examination were performed. The levels of tumor necrosis factor（ TNF）-α,interleukin（ IL）-1β,and IL-6 in the inflamed paw tissues were measured. Results The administration of CAI significantly decreased the AI,restored the body weights,and ameliorated the radiological and histopathological features of joint destruction in AA rats（ P〈0. 05,P〈0. 01）. In addition,CAI reduced the TNF-α,IL-1β,and IL-6 levels in the inflamed paw tissues（ P〈0. 05,P〈0. 01）. Conclusion CAI has therapeutic effect on AA rats,which may be achieved by decreasing the pro-inflammatory cytokines at the site of inflammation.

关 键 词：羧胺三唑 佐剂性关节炎 细胞因子 

分 类 号：R961[医药卫生—药理学]