胶原温敏半互穿水凝胶的制备及其对L929细胞行为的影响  

Thermo-sensitive Semi-interpenetrating Collagen Hydrogels and Effects on L929 Cell's Behavior

作　　者：张浩[1] 胡晓婷[1] 杨宁[1] 陈莉[1] 

机构地区：[1]天津工业大学材料科学与工程学院,天津300387

出　　处：《高分子学报》2016年第3期300-306,共7页Acta Polymerica Sinica

基　　金：国家自然科学基金(基金号31200719;50973084)资助项目

摘　　要：以Ⅰ型胶原和N-异丙基丙烯酰胺(NIPAAm)为主要原料,将Ⅰ型胶原引入到PNIPAAm交联网络中,制得一种具有温度响应性的半互穿水凝胶.通过红外光谱(FTIR)和扫描电镜(SEM)对PNIPAAm/CollagenⅠ半互穿水凝胶进行成分和结构的表征;通过溶胀测试和示差扫描量热法(DSC)研究了半互穿水凝胶的温敏特性,并对其表面亲疏水性进行分析;在水凝胶表面培养L929细胞,研究其增殖脱附行为.结果表明,PNIPAAm/CollagenⅠ半互穿水凝胶具有良好的温度响应性和生物相容性,与PNIPAAm水凝胶相比,PNIPAAm/CollagenⅠ半互穿水凝胶表面更有利于L929细胞的黏附增殖.将温度降至临界温度(LCST,32℃)以下,细胞从凝胶表面自发脱附.细胞染色表明,与胰蛋白酶消化相比,降温脱附的细胞损伤少,活性更高,表明PNIPAAm水凝胶中引入胶原后,生物相容性得到改善.The thermo-sensitive collagen hydrogels with semi-interpenetrating polymer network（ semi-IPN）were successfully prepared by inducing type Ⅰ collagen into poly（ N-isopropylacrylamide） cross-linked network. The chemical composition and pore structure of the semi-IPN gels were characterized by Fourier transform infrared spectroscopy（ FTIR） and scanning electron microscopy（ SEM）. Swelling ratio measurements and differential scanning calorimetry（ DSC） were used to investigate the thermo-sensitivity of hydrogels and contact angles were studied to analyze gels surface hydrophilicity. Additionally,L929 cells ＇behaviors such as adhesion,proliferation and detachment were carefully studied via cell culture on the surface of PNIPAAm/CollagenⅠ semi-IPN hydrogels in vitro. The results indicated that the PNIPAAm/Collagen Ⅰsemi-IPN hydrogels present excellent thermo-sensitivity and biocompatibility. Cell cultures of L929 on the semiIPN hydrogels showed better proliferation and viability than on the PNIPAAm hydrogel. By lowering temperature below the lower critical solution temperature（ LCST）,L929 cells could spontaneously detach from the surface of gels. Meanwhile,the detached cells were collected for trypan blue staining and hematoxylin and eosin staining. Results showed that extracellular matrix proteins and trans-membrane proteins were hydrolysed by trypsin digestion. Cells spontaneously detached from the semi-IPN hydrogels showed higher viability than those from PNIPAAm hydrogel,which suggesting that the semi-IPN hydrogels have potential value in tissue engineering.

关 键 词：胶原 PNIPAAM 半互穿水凝胶 细胞行为 温敏性 

分 类 号：Q2-33[生物学—细胞生物学] O648.17[理学—物理化学]