模拟人体尿道插管法行小鼠尿动力学检测  

Simulation of human urethral catheterization to implement urodynamic testing in mice

作　　者：陈林[1] 杨进[1] 胡海峰[1] 邢莎莎[2] 张汉超[1] 喻备[1] 杨亚飞[1] 

机构地区：[1]成都大学附属医院泌尿外科,四川成都610081 [2]成都大学附属医院中心实验室,四川成都610081

出　　处：《中国病理生理杂志》2016年第2期381-384,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81500577);四川省卫生厅课题(No.090112;No.100073);成都大学青年基金资助项目(No.2080515062)

摘　　要：目的:国际上传统的小鼠尿动力学检测方法为膀胱造瘘法。该传统方法破坏膀胱连续性,与临床上广泛采用的经尿道法有明显的差异。本研究寻找到合适的小鼠导尿管材,拟探讨模拟人体经尿道插管法行小鼠尿动力学检测的优势和应用价值。方法:雌性小鼠8只,选择小儿留置针软芯进行导尿,并作为测压管和膀胱灌注管,经三通外接微量泵以及压力感受器。直肠测压采用硬膜外麻醉导管,外套乳胶手套小指末端一截制成球囊,然后导入小鼠直肠。结果:该方法成功收集到以下尿动力学指标:膀胱基础压力(BBP)、膀胱漏尿点压(BLPP)、最大排尿压(MVP)、膀胱最大容量(MBC)、残余尿量(PVR)、排尿量(VV)、排尿效率(EV)和膀胱顺应性(BC)。结论:本研究首次成功模拟人体经尿道插管法,实现小鼠尿动力学检测,成功避免膀胱造瘘对小鼠尿动力学的影响,检测后的小鼠能够长期生存,可以顺利进行后续分子和基因实验。AIM： Cystostomy is the traditionary method for detecting urodynamic indexes in mice, which de- stroys the continuity of the bladder, and there are significant differences between this method and the clinically used trans- urethral method. This study aims to develop an appropriate urethral catheter to investigate the advantages and application val- ue of transurethral method for urodynamic test. METHODS ： A pediatric intravenous catheter was used for urethral catheter- ization on 8 female mice, and linked to connect the catheter to baroreceptor and micropump. The epidural catheter was also used as manometry tube. RESULTS： Using this method, the following urodynamic indicators has been successfully cap- tured： basal bladder pressure （BBP）, bladder leak point pressure （BLPP）, maximum voiding pressure （MVP）, maxi- mum bladder capacity （ MBC ） , post-void residual urine volume （ PVR）, voiding volume （ VV ） , efficiency of voiding （EV） and bladder compliance （BC）. CONCLUSION： This is the first successful simulation used in human body to a- chieve mouse urodynamic testing through the urethra catheter, which avoids the impact of cystostomy on urodynamics in mice, and the mice are able to keep long-term survival after tests for the follow-up molecular and genetic experiments.

关 键 词：小鼠 尿动力学 膀胱造瘘术 尿道插管术 

分 类 号：R33-33[医药卫生—人体生理学]