燃煤型氟中毒对仔鼠氟斑牙和氧化应激的影响  

Effects of coal- burning endemic fluorosis on the dental fluorosis and oxidative stress of the rats offspring

作　　者：初可嘉 王海慧 白国辉[2] 管晓燕[2] 刘建国[2] 

机构地区：[1]贵州市口腔医院,贵州贵阳550002 [2]遵义医学院口腔医院,贵州遵义563000

出　　处：《现代预防医学》2016年第5期806-809,共4页Modern Preventive Medicine

基　　金：贵州省第六批科技创新人才团队建设项目(合同号:黔科合人才团队[2013]4026号);贵州省高等学校重点学科建设项目(合同号:SZXK-2012-07-04);贵州省高等学校特色重点实验室建设项目(合同号:黔教合KY字[2013]109号);省市科技合作专项资金项目(合同号:省市科合[2014]41号);贵州省卫生计生委科学技术基金项目(合同号:gzwjkj2014-1-021号);贵阳市高层次创新型青年卫生人才培养计划项目(合同号:201503)

摘　　要：目的探讨燃煤型氟中毒对仔鼠氟斑牙发生和氧化应激的影响。方法模拟病区燃煤方式,复制高氟空气环境,疫区煤烘玉米制作含氟饲料。48只SD大鼠按雌雄比例2:1随机分为4组,即高氟空气饲养室内分别给予饲料氟含量40mg/kg(高氟组)、25mg/kg(中氟组)、10mg/kg(低氟组)和阴性对照组。饲养8周后合笼交配。仔鼠出生后21天(哺乳期)观察下切牙氟斑牙发生情况,并检测血清中丙二醛(MDA)含量和超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物(GSH-Px)活性。结果出生后21天,高氟组、中氟组仔鼠下切牙釉质出现棕白相间横纹等典型氟斑牙症状。与对照组相比,各实验组仔鼠血清中MDA含量升高(F=796.229,P<0.001),SOD、CAT活性降低(F=119.315、P<0.001;F=133.670,P<0.001),且随着亲代染氟剂量的增加呈加重趋势;GSH-Px活性变化无统计学意义(F=1.666,P=0.188)。结论亲代大鼠摄入燃煤来源过量氟可以导致仔鼠氟斑牙的发生和血清氧化-抗氧化系统失衡,氧化应激在氟斑牙发生过程中可能起重要作用。Objective To research the effects of overdose fluoride caused by coal- burning on dental fluorosis and oxidative stress of the rats offspring. Methods High fluoride air model was established on the basis of burning coal habit of epidemic area. Fluoride feed was made of coal dried corn. 48 SD rats were randomly divided into 4 groups,in the high fluoride air room and feed a food with fluorine at 40 mg / kg（ high fluoride group）,25 mg / kg（ middle fluoride group）,10 mg / kg（ low fluoride group） and the control group. The female to male ratio was 2： 1. After eight weeks,the male and female mated with each other. At the 21 d,dental fluorosis of the lower incisors was observed,and then the contents of MDA and the activities of SOD,CAT,GSH- Px were measured in serum of rats offspring. Results The typical symptom which was a succession of white and brown stripes of dental fluorosis was observed in the high fluoride group and middle fluoride group. The contents of MDA of the experimental groups increased（ F = 796. 229,P〈 0. 001） while the activities of SOD and CAT decreased（ F = 119. 315,P〈 0. 001; F = 133. 670,P〈 0. 001）,and the symptom increased with the dose of fluorine. There was no significant difference in the activities of GSH- Px（ F = 1. 666,P = 0. 188）. Conclusion The high fluoride environment has an effect on the dental fluorosis. The balance of oxidative and anti- oxidative system and oxidative stress of the rats offspring may play an important role in the dental fluorosis process.

关 键 词：燃煤型氟斑牙 氧化应激 仔鼠 氟中毒 

分 类 号：R12[医药卫生—环境卫生学]