免疫毒理学体外替代方法研究进展  

Advances in in Vitro Alternative Methods of Immunotoxicology

作　　者：陈虹[1] 王春仁[1] 周小婷 张梅玲[3] 韩倩倩[1] 赵丹妹[1] 

机构地区：[1]中国食品药品检定研究院,北京100050 [2]河南医疗器械检验所,郑州450003 [3]中国药科大学,南京211198

出　　处：《中国医疗器械信息》2016年第2期29-34,55,共7页China Medical Device Information

摘　　要：传统上,我们使用动物试验来推测施用于人体的材料或产品等可能给人体带来的免疫毒性危害。但由于动物与人类种属差异的局限性和动物保护福利要求的日益加强,传统的动物实验已经不能满足对毒理学安全性评价的要求。寻找与人类的在体实验原理更接近并且高效、灵敏又快速的体外毒理学安全性评价方法成为近几年国际上的研究热点。随着基础科研研究的不断深入,免疫毒理的一些机理、细胞通路、毒理学终点日渐被发现并被认知,科研工作者们开发了一些体外替代方法逐步取代动物实验。本文介绍若干免疫毒理学体外替代方法的研究进展,通过比较各方法优点及局限性给拟使用这些方法进行毒理评价的工作者一些参考意见。Traditionally, we use animal test to speculate possible immunotoxicity harm when we apply materials or products to the human body. However, due to limitations caused by animal and human species difference and increasingly animal welfare protection requirements traditional animal toxicology experiments can not meet the requirements of safety evaluation. Looking for alternative experiment methods which are more closer to mechanisms of human body and more efficient, sensitive or rapid for in vitro toxicology safety evaluation studies in recent years become an international popular issue. With the deepening of basic research studies, some immunotoxicity mechanisms, cellular pathways, toxicological endpoints increasingly being discovered.Researchers have developed a number of in vitro alternatives to replace animal experiments gradually. This article describes some advances in immunotoxicology alternative methods in vitro, giving some suggestions by comparing the advantages and limitations of these methods.

关 键 词：免疫毒理 动物试验 体外替代方法 

分 类 号：R994.1[医药卫生—毒理学]