罗氏海盘车(Asterias rollestoni)中2种生物碱的分离纯化及其抗肿瘤活性研究  

The preparation and purification of two alkaloids derived from Asterias rollestoni and studies on their antitumor activities

作　　者：宋龙[1] 吕律[1] 王远红[1] 姜廷福[1] 吕志华[1] 

机构地区：[1]中国海洋大学海洋药物教育部重点实验室,医药学院,山东青岛266003

出　　处：《中国海洋药物》2016年第1期50-54,共5页Chinese Journal of Marine Drugs

基　　金：山东省海洋经济创新发展区域示范项目(鲁财建指[2013]361号)资助

摘　　要：目的从罗氏海盘车(Asterias rollestoni)中分离鉴定生物碱类天然产物,并进行体外抗肿瘤活性评价。方法利用凝胶吸附树脂柱层析、硅胶薄层层析、硅胶柱层析、凝胶Sephadex LH-20柱层析和高效液相色谱等手段对化合物进行了分离纯化;运用NMR、MS等波谱方法并结合文献鉴定化合物的结构;采用SRB、MTT法评价化合物的体外抗肿瘤活性。结果从罗氏海盘车的乙醇提取物中分离鉴定了1个吲哚生物碱N-(2-苯乙基)-1H-吲哚-3-乙胺(1)和1个环二肽生物碱类化合物Fellutanine A(2)。化合物2对MGC803细胞的抑制率为53.99%,具有一定的抗肿瘤活性。结论 2个生物碱类化合物均为首次从罗氏海盘车中分离获得;化合物1为首次分离到的天然产物,其核磁数据为首次报道;化合物2具有一定的抗肿瘤活性。Objective To isolate and identify alkaloids from Asterias rollestoni and evaluate their antitumor activities.Methods Isolation and purification were performed by MCI GEL CHP20 gel adsorption resin,TLC,silica gel column chromatography,Sephadex LH-20 column chromatography,and HPLC.Structural characterization was established by spectroscopic analysis（NMR,MS）and comparison with literature data.Antitumor activities were evaluated by SRB and MTT methods.Results Two alkaloids were isolated and identified as N-（2-phenylethyl）-1H-Indole-3-ethanamine（1）and Fellutanine A（2）.Compound 2 exhibited potent cytotoxic activity against MGC803 with the inhibition rate of 53.99%.ConclusionIt＇s the first report for both alkaloids isolated fromAsterias rollestoni.Compound1 was first isolated as natural product and its NMR spectroscopic data was first reported.Compound 2 exhibited potent cytotoxic activity against cancer cell.

关 键 词：罗氏海盘车 生物碱 抗肿瘤活性 非核糖体肽 

分 类 号：R931[医药卫生—生药学]