基于流式细胞术研究管网水中细菌的稳定性  

Monitoring the bacterial stability in drinking water distribution systems by flow cytometry

作　　者：刘婷婷[1] 陈南[1] 孔维文[1] 王敬琦[1] 何晓青[1] 

机构地区：[1]北京林业大学生物科学与技术学院,北京100083

出　　处：《应用与环境生物学报》2016年第1期146-150,共5页Chinese Journal of Applied and Environmental Biology

基　　金：中央高校基本科研业务费专项(TD2012-03);水利部行业公益性行业科研专项(201201032);国家自然科学基金青年科学基金项目(51108029)资助~~

摘　　要：为了更准确地研究管网水中细菌的稳定性,利用流式细胞术（Flow cytometry,FCM）和异养菌平板计数法（Heterotrophic plate count,HPC）检测北京市12处管网末梢水中细菌数量,并将两种方法的检测结果进行对比.进一步将FCM应用于细菌的再生长潜能和一天中细菌稳定性的研究中.结果显示,HPC对12处管网水中细菌的检测结果在0-23 CFU/m L间,而FCM测得活细菌数为2.2×10^3-1.6×10^4 cells/m L,细菌总数在22℃下培养7 d能生长到10^6-10^7 cells/m L.一天中不同时间用水量不同,细菌总数也不同.本研究表明,FCM测定方法明显优于HPC法,且能够快速准确地反映细菌的再生长潜能及短时间内细菌的稳定性特征.This research investigated the drinking water distribution system of Beijing for the bacterial stability in chlorinated drinking water.Flow cytometry（FCM） and heterotrophic plate counts（HPC） methods were used to evaluate the drinking water quality and stability of 12 water samples throughout the distribution network.The samples were incubated at 22 ℃without shaking;total cell counts and percentages of intact cells measurements were done by FCM daily during 7 consecutive days.Another detailed assessment of dynamic changes was done at 1-hour intervals at 8 am- 8 pm in each day.The results showed FCM intact cell concentrations varying from 2.2 × 10^3 cells/mL to 1.6×10^4 cells/mL in the network,while HPC results varying from 0 CFU/mL to 23 CFU/mL.The average total cell counts at the end of the 7-day cultivation for all samples were approximately 10^6- 10^7 cells/mL.High frequency monitoring of a single sampling point demonstrated temporal instability in the network.This research obtained some data by FCM that was not detectable by HPC.We understand it as that FCM is suitable for monitoring the biological stability and regrowth potential of bacteria in drinking water systems.

关 键 词：管网水 流式细胞术 再生长潜能 生物稳定性 

分 类 号：Q93[生物学—微生物学]