代谢组学在小鼠变应性鼻炎诊断中的实验研究  

Experimental study of metabonomics in the diagnosis of allergic rhinitis in mice

作　　者：王媛[1] 李青峰[1] 张高勤 赵长青[1] 

机构地区：[1]山西医科大学第二医院耳鼻咽喉头颈外科,太原030001 [2]山西省公安厅理化检验实验室,太原030001

出　　处：《中华耳鼻咽喉头颈外科杂志》2016年第2期124-127,共4页Chinese Journal of Otorhinolaryngology Head and Neck Surgery

基　　金：国家自然科学基金（81271059）;山西省归国留学人员基金（20130313022-1）

摘　　要：目的 探讨代谢组学在变应性鼻炎（allergic rhinitis,AR）小鼠动物模型中的诊断作用.方法 雄性昆明小鼠80只,随机数字表法分成2组,对照组30只,AR组50只.建模后剔除过敏症状行为学评分记录叠加总分＞6分者,保留症状行为学评分叠加总分为6分的30只AR小鼠.收集各组小鼠外周血,取血清液,采用超高效液相色谱-串联质谱法（UPLC-MS）分离检测,利用MZmine软件进行色谱峰匹配后,用SPSS及Excel软件对数据进行预处理,按照80％法则删除干扰数据,通过PCA-X和PLS-DA分析考察数据的特征.结果 取AR组叠加计分为6分的30只小鼠为研究对象.对照组（30只）和AR组（30只）小鼠血清代谢物检测数据用PCA-X、PLS-DA法建模后绘制的视图,两组样本可以达到基本分离,说明两组数据差异存在统计学意义,即对照组和AR组小鼠血清代谢物组存在一定差异.结论 利用代谢组学进行小鼠AR实验动物的诊断具有一定的可行性.Objective To investigate the application of metabonomics in the diagnosis of allergic rhinitis.Methods Eighty male Kunming mice were randomly divided into two groups,control group （30 mice） and allergic rhinitis （AR） group （50 mice）.After modeling,removal behavior score more than 6 and retain 30 mice behavior score equal to 6.Collect the mice peripheral blood and preparate blood serum,using UPLC-MS chromatographic separation and detection.The data were pretreated by SPSS and Excel,after chromatographic peak matching by MZmine.Firstly,delete interference data in accordance with the 80% rule.Then,the investigate data were analyzed by PLS-DA and PCA-X.Results Three-dimensional view of the control group （30 mice） and AR group （30 mice） blood serum data was drawn using PCA-X and PLS-DA method.The two groups of samples could be completely separated through views,which showed that there was a significant difference between the two groups of data.There were some differences in the blood metabolites between the control group and AR group.Conclusion The study showed that it was scientific and feasible to diagnose AR using the metabonomics.

关 键 词：鼻炎 变应性 常年性 疾病模型 动物 代谢组学 诊断技术和方法 

分 类 号：R992[医药卫生—毒理学]