针刀干预对LDH根性痛大鼠背根神经节内P物质和降钙素基因相关肽的影响  

Effect of Acupotomy on the Substance P and Calcitonin Gene- related Peptide in the Dorsal Root Ganglion of Rats with Radicular Pain of Lumbar Disc Herniation

作　　者：张伟夫[1] 郭长青[1] 张义[1] 王美琴[1] 何智菲 孙红梅[1] 许红[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《针灸临床杂志》2016年第2期60-64,101,共6页Journal of Clinical Acupuncture and Moxibustion

基　　金：北京中医药大学自主课题项目;编号:2015-JYB-XS128

摘　　要：目的:观察针刀干预对腰椎间盘突出症(LDH)根性痛大鼠背根神经节内P物质(SP)和降钙素基因相关肽(CGRP)含量的影响。方法:将32只雄性SD大鼠随机分为空白组、模型组、电针组和针刀组,每组8只。在造模后第8天,对各组大鼠进行心脏灌注,并取出各组大鼠L5背根神经节,再采用双抗夹心ELISA方法检测各组大鼠背根神经节中CGRP和SP的含量。结果:各组大鼠L5背根神经节中SP含量的比较,模型组(19.70±1.63)较空白组(14.42±1.71)显著升高,差异有统计学意义(P<0.01),针刀组(15.08±2.07)较模型组(19.70±1.63)显著降低,差异有统计学意义(P<0.01);CGRP含量的比较,模型组(22.43±2.14)较空白组(19.84±2.91)显著升高,差异有统计学意义(P<0.05),针刀组(19.36±2.79)较模型组(22.43±2.14)显著降低,差异有统计学意义(P<0.05)。结论:针刀疗法可改善LDH大鼠根性痛症状,它可能通过调节背根神经节内SP和CGRP的含量变化而发挥其治疗作用。Objective： To observe the effect of acupotomy on the changes of SP and CGRP in the dorsal root ganglion（ DRG） of rats with radicular pain of lumbar disc herniation. Methods： Thirty- two male SD rats were randomly divided into a blank group,a model group,an acupotomy group and an electroacupuncture group,with eight rats in each group. On the eighth day after modeling,all the rats were performed with heart perfusion,and the DRG was taken to measure the contents of SP and CGRP by ELISA. Results： The comparison of SP among each group was that the model group（ 19. 70 ± 1. 63） was increased significantly（ P 〈0. 01） compared with the blank group（ 14. 42 ± 1. 71）; acupotomy group（ 15. 08 ± 2. 07） was decreased significantly compared with the model group（ 19. 70 ± 1. 63）（ P 〈0. 01）. The comparison of CGRP among each group was that the model group（ 22. 43 ± 2. 14） was increased（ P 〈0. 05） compared with the blank group（ 19. 84 ± 2. 91）; acupotomy group（ 19. 36 ± 2. 79） was decreased compared with the model group（ 22. 43 ± 2. 14）（ P 〈0. 05）. Conclusion： Acupotomy therapy can improve the symptom of radicular pain of rats with lumbar disc herniation,and it may be by regulating the SP and CGRP in DRG to achieve.

关 键 词：针刀 腰椎间盘突出症 根性痛 背根神经节 SP CGRP 

分 类 号：R246.6[医药卫生—针灸推拿学]