荷叶炒炭过程中金丝桃苷、异槲皮苷与槲皮素含量变化规律研究  

Study on Content Variations of Hyperoside,Isoquercitrin and Quercetin in Fried Charcoal Lotus Leaf

作　　者：李坤[1] 王明芳[1] 薛非非 国金铃 马俊楠[1] 孟祥龙[1] 张朔生[1] 

机构地区：[1]山西中医学院制药与食品工程学院,晋中030619

出　　处：《世界科学技术-中医药现代化》2016年第1期112-117,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：山西省研究生优秀创新项目(20133123):基于热分析技术对炒炭工艺与"炒炭存性"相关性研究;负责人:马俊楠;山西中医学院研究生创新基金(YCX-1408):荷叶不同炮制品药理作用与物质基础相关性研究;负责人:李坤

摘　　要：目的:本研究主要探究不同炒炭程度对荷叶炭中金丝桃苷、异槲皮苷与槲皮含量变化的影响,并建立HPLC同时测定这3种物质的方法,通过模拟炮制来研究这3种物质转变的关系。方法:本实验采用Hypersil gold C18(4.6 mm×250 mm,5μ)色谱柱;以乙腈(A)-0.1%磷酸(B)为流动相,梯度洗脱;流速:1.0 mL·min^(-1);检测波长:360 nm;柱温:35℃。结果:荷叶炒炭后,金丝桃苷与异槲皮苷含量随炮制时间呈现逐渐下降趋势;槲皮素含量随着炮制时间呈现先上升、后下降趋势,并在280±5℃温度下炒制10 min左右达到峰值;另外,在金丝桃苷和异槲皮苷模拟炮制样品中均检出槲皮素。结论:该实验说明不同炒炭程度对荷叶炭中金丝桃苷、异槲皮苷与槲皮素含量有显著影响,在加热炮制过程中金丝桃苷和异槲皮苷受热分解产生槲皮素。This study was aimed to analyze the content changes of hyperoside,isoquercitrin and quercetin in charred lotus leaf at different fried charcoal processing degrees.The simultaneous determination on 3 substances by HPLC was established.The material change relation among 3 substances was studied through the simulated processing.Hypersil gold C_（18） column（4.6 mm×250mm,5μm） was used in the study.The mobile phase was acetonitrile（A）-0.1%phosphoric acid（B） with gradient elution.The flow speed was 1.0 mL·min^-1.The detection wavelength was 360 nm.The column temperature was 351.The results showed that after the lotus leaf was fried charcoal,the contents of hyperoside and isoquercitrin decreased gradually along with the processing time.The content of quercetin increased first and then decreased with the increase of processing time.The content of quercetin in lotus leaf achieved the highest when it was processed for 10 min at the temperature of 280±℃.Quercetin was detected in both the hyperoside and isoquercitrin simulated processing samples.It was concluded that different processing degrees of fried charcoal lotus leaf had remarkable effects on the contents of hyperoside,isoquercitrin and quercetin.During the heating process,quercetin was decomposed from both hyperoside and isoquercitrin.

关 键 词：荷叶 炒炭 黄酮 高效液相 模拟炮制 炮制机理 

分 类 号：R943.1[医药卫生—药剂学]