HPLC测定FKⅣ号颗粒中芍药苷、绿原酸、虎杖苷及丹酚酸B的含量  

Content Determination of Paeoniflorin,Chlorogenic Acid,Polydatin and Salvianolic Acid B in FK IV Granules by HPLC

作　　者：孙仙玲[1,2] 秦建平[3,2] 王宪平[3,2] 石伟[1,2] 钱俊[3,2] 吴云[3,2] 王振中[3,2] 萧伟[1,3,2] 

机构地区：[1]南京中医药大学药学院,南京210000 [2]中药制药过程新技术国家重点实验室,连云港222001 [3]江苏康缘药业股份有限公司,连云港222001

出　　处：《世界科学技术-中医药现代化》2016年第1期125-129,共5页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：科学技术部国家重大新药创制专项(2013ZX09402203):FKⅣ号颗粒的药学研究;负责人:王振中

摘　　要：目的:建立同时检测FKⅣ号颗粒中芍药苷、绿原酸、虎杖苷及丹酚酸B的HPLC含量测定方法。方法:采用HPLC含量测定法,色谱柱为Kromasil C18(4.6 mm×250 mm,5μm);以乙腈-0.1%磷酸水溶液为流动相,梯度洗脱,芍药苷检测波长为230 nm,绿原酸、虎杖苷、丹酚酸B检测波长为306 nm;柱温30℃,进样量10μL。结果:芍药在7.539-482.470μg·mL^(-1)范围内呈良好线性关系(r=1.000),平均回收率为101.10%;绿原酸在2.003-128.166μg·mL^(-1)范围内呈良好线性关系(r=1.000),平均回收率为100.76%;虎杖苷4.503-288.200μg·mL^(-1)范围内呈良好线性关系(r=1.000),平均回收率为100.74%;丹酚酸B在4.621-295.752μg·mL^(-1)范围内呈良好线性关系,平均回收率为99.91%(r=0.999 8)。结论:该方法简便可靠,重复性好,可作为FKⅣ号颗粒中芍药苷、绿原酸、虎杖苷及丹酚酸B的含量测定方法。This study was aimed to establish the simultaneous content determination of paeoniflorin,chlorogenic acid,polydatin and salvianolic acid B in FK Ⅳ granules by HPLC.The Kromasil C18 column（4.6 mm×250 mm,5Jim） was used as chromatographic column.The acetonitrile-0.1%phosphoric acid solution was used as mobile phase with gradient elution.The detection wavelength of paeoniflorin was 230 nm.And the detection wavelength of chlorogenic acid,polydatin and salvianolic acid B was 306 nm.The column temperature was 30℃.The sample injection volume was 10μL.The results showed that paeoniflorin had a good linear relationship in the range of7.539-482.470μg·mL^-1（r = 1.000） with the average recovery rate of 101.10%.Chlorogenic acid showed a good linear relationship in the range of 2.003-128.166μg·mL^-1（r = 1.000） with the average recovery rate of 100.76%.Polydatin showed a good linear relationship in the range of 4.503-288.200μg·mL^-1（r = 1.000） with the average recovery rate of 100.74%.Salvianolic acid B showed a good linear relationship in the range of 4.621-295.752μg·mL^-1（r=0.999 8） with the average recovery rate of 99.91%.It was concluded that the method was simple and reliable with good reproducibility.It can be used as a method for the content determination of paeoniflorin,chlorogenic acid,polydatin and salvianolic acid B in FK Ⅳ granules.

关 键 词：FKⅣ号颗粒 HPLC 芍药苷 绿原酸 虎杖苷 丹酚酸B 

分 类 号：R284[医药卫生—中药学]