皮秒脉冲电场抑制人脐静脉内皮细胞增殖和迁移并阻滞细胞周期于G2/M期且促进其凋亡  

Picosecond pulsed electric field inhibits the proliferation and migration,induces G2/M phase arrest and promotes apoptosis in human umbilical venous endothelial cells

作　　者：吴雨桐[1] 华媛媛[1] 熊正爱[1] 吴丽梅[1] 姚陈果[1] 张睿哲[1] 王智亮[1] 

机构地区：[1]重庆医科大学附属第二医院,重庆400010

出　　处：《细胞与分子免疫学杂志》2016年第3期319-322,327,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81301928;81201745);重庆市卫生局资助项目(2011-2-155;2012-2-068)

摘　　要：目的研究皮秒脉冲电场(ps PEF)对体外人脐静脉内皮细胞(HUVEC)生物学行为的影响。方法固定ps PEF脉宽800 ps、频率3 Hz、脉冲处理个数2000个,将HUVEC分为对照组和(200、300、400)KV/cm场强的低、中、高剂量处理组。在ps PEF处理HUVEC后2、4、6、8、10、12、14、16 h,CCK-8法检测ps PEF对HUVEC生长的影响,采用划痕试验及TranswellTM小室法观察ps PEF对HUVEC迁移率的影响,流式细胞术检测ps PEF对HUVEC细胞周期及凋亡的作用。结果随着场强的增加,细胞死亡率上升,生长受到抑制,12 h时抑制率最为显著;ps PEF处理可明显抑制HUVEC的迁移能力;ps PEF可将HUVEC阻滞于G2/M期,同时HUVEC凋亡细胞数增多。结论 ps PEF可抑制HUVEC增殖和迁移,并阻滞细胞周期于G2/M期并促进其凋亡。Objective To study the impact of intense picosecond pulsed electric field（ ps PEF） on the biological behaviors of human umbilical venous endothelial cells（ HUVECs） in vitro. Methods Intense ps PEF with constant parameters（ pulse duration,800 ps; recurrence rate,3 Hz; the number of pulses,2000） and three-level electric field strength（ 200,300 and400 KV / cm） were performed on HUVECs. CCK-8 assay was used to detect the cell proliferation of HUVECs treated with ps PEF for 2,4,6,8,10,12,14,16 hours. The effect of ps PEF on HUVEC migration was measured by wound healing method and TranswellTMassay. The apoptosis and cell cycle were detected by flow cytometry. Results The death rate of HUVECs went up with the increase of the amount of pulses,and the maximum inhibitory rate was at 12 hours after the treatment with ps PEF. Intense ps PEF inhibited dose-dependently the migration of HUVECs. What＇s more,the treatment with ps PEF arrested cell cycle in G2 / M phase and increased the apoptosis rate. Conclusion Intense ps PEF inhibits the migration and proliferation of HUVECs as well as induces cell apoptosis and arrests the cell cycle in G2 / M phase.

关 键 词：皮秒脉冲电场 人脐静脉内皮细胞 血管生成 

分 类 号：P319.12[天文地球—固体地球物理学] R392-33[天文地球—地球物理学]