姜黄素改善蛛网膜下腔出血大鼠学习记忆功能并抑制海马组织TNF-α和iNOS水平上升  

Curcumin improves learning and memory function through decreasing hippocampal TNF-α and i NOS levels after subarachnoid hemorrhage in rats

作　　者：仇振巍[1] 岳双柱[2] 

机构地区：[1]濮阳市安阳地区医院神经外一科,河南安阳455000 [2]新乡医学院第一附属医院神经外科,河南卫辉453100

出　　处：《细胞与分子免疫学杂志》2016年第3期343-346,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81300956)

摘　　要：目的研究姜黄素对大鼠蛛网膜下腔出血(SAH)模型学习记忆功能的影响及可能机制。方法 30只SD大鼠随机分为对照组、SAH组和姜黄素治疗组,利用自体血液注射的方法制备大鼠SAH模型,利用神经功能评分分析模型情况,Morris水迷宫实验检测各组大鼠的学习记忆功能,ELISA检测各组大鼠海马组织肿瘤坏死因子α(TNF-α)和诱导型一氧化氮合酶(i NOS)的含量。结果 SAH模型动物神经功能评分显著低于正常对照组,姜黄素治疗组大鼠神经功能评分显著得到改善。Morris水迷宫检测结果表明,与对照组相比,SAH大鼠平均逃避潜伏期延长,经姜黄素治疗后,平均逃避潜伏期明显降低。ELISA检测结果显示,与假手术组相比,SAH大鼠海马组织中TNF-α和i NOS含量显著增加,给予连续4周的姜黄素治疗后,大鼠海马组织中TNF-α和i NOS明显下降。结论姜黄素治疗可以改善SAH大鼠学习记忆功能,降低SAH大鼠海马TNF-α及i NOS水平。Objective To investigate the effect of curcumin on learning and memory function of rats with subarachnoid hemorrhage（ SAH） and the possible mechanism. Methods A total of 30 male Sprague-Dawley rats were randomly divided into three groups： Sham group,SAH group and curcumin（ Cur） therapy group. Experimental SAH rat models were established by injecting autologous blood into the cisterna magna. Neurological deficits of rats were examined at different time points.Spatial learning and memory abilities were tested by Morris water maze test. The hippocampal tumor necrosis factor-alpha（ TNF-α） and inducible nitric oxide synthase（ i NOS） were detected by ELISA. Results Experimental SAH rat models were established successfully. Neurological scores of the SAH rats were significantly lower than those of the sham group.Curcumin therapy obviously improved the neurological deficits of rats compared with the SAH rats. Morris water maze test showed that SAH caused significant cognitive impairment with longer escape latency compared with the sham group. After treatment with curcumin for 4 weeks,the escape latency decreased significantly. The levels of TNF-α and i NOS in the curcumin-treated group were significantly lower than those of the SAH group. Conclusion SAH can cause learning and memory impairment in rats. Curcumin can recover learning and memory function through down-regulating hippocampal TNF-α and i NOS levels.

关 键 词：蛛网膜下腔出血 肿瘤坏死因子α 诱导型一氧化氮合酶 海马 姜黄素 学习记忆 

分 类 号：R722.151[医药卫生—儿科] R743.34[医药卫生—临床医学]