长春地区儿童疱疹性咽峡炎病原研究  

Pathogenic study on herpangina among children in Changchun

作　　者：许爽[1] 刘金香[2] 吴东林[1] 杨显达[1] 魏雷雷[1] 李响[1] 

机构地区：[1]吉林省疾病预防控制中心,吉林长春130062 [2]吉林大学第一医院,吉林长春130031

出　　处：《中国卫生检验杂志》2016年第3期391-393,共3页Chinese Journal of Health Laboratory Technology

基　　金：国家科学技术艾滋病和病毒性肝炎等重大传染病防治专项传染病监测技术平台项目(2012ZX10004209-003)

摘　　要：目的了解长春地区2012年、2013年儿童疱疹性咽峡炎主要病原类型。方法采集2012年、2013年儿童疱疹性咽峡炎患者的咽拭子标本,通过半巢式RT-PCR及VP1区基因序列分析进行病原分型鉴定。结果本研究采集的110份儿童疱疹性咽峡炎标本中,经鉴定肠道病毒(enterovirus,EV)阳性82份,阳性率为74.55%;其中2012年检测前3位的型别分别为CVA10(18/32)、CVA2(7/32)及CVA3、CVA16(3/32);2013年检测前3位的型别分别为CVA6(25/50)、CVA2(13/26)及CVA4、CVA10(3/50)。系统进化树分析结果表明,2012年和2013年吉林省肠道病毒的流行均存在3个或3个以上的传播链。结论 2012年、2013年引起长春地区儿童疱疹性咽峡炎的主要病原分别为CVA10及CVA6,且同期存在不同的传播链。Objective To investigate the major pathogens of angina among children in Changchun during 2012- 2013. Methods Throat swabs of angina among children during 2012- 2013 were collected,and semi- nested RT- PCR and VP1 region analysis were conducted for pathogen typing identification. Results Among the 110 cases of angina specimens collected from children,82 cases were identified as enterovirus- positive,with the positive rate of 74. 55%. In 2012,the top 3 types in detection were CVA10（ 18 /32）,CVA2（ 7 /32）,CVA3 and CVA16（ 3 /32）; in 2013,the top 3 types were CVA6（ 25 /50）,CVA2（ 13 /26）,CVA4 and CVA10（ 3 /50）,respectively. The analysis results of phylogenetic tree showed that there were 3 or more than 3 transmission chains on the prevalence of enteroviruses in Jilin in 2012 and 2013. Conclusion CVA10 and CVA6 were major pathogens causing herpangina among children in Changchun in 2012 and 2013,and there were different transmission chains in the same period.

关 键 词：疱疹性咽峡炎 柯萨奇A10型病毒 柯萨奇A6型病毒 

分 类 号：R725.1[医药卫生—儿科]