隐丹参酮下调U2OS骨肉瘤细胞血管内皮生长因子表达并抑制血管生成  

Cryptotanshinone down-regulates the expression of VEGF and inhibits angiogenesis in U2OS osteosarcoma cells

作　　者：徐亚梦 冀全博[2] 张强[2] 王岩[2] 

机构地区：[1]上海交通大学医学院附属新华医院中医科,上海200092 [2]解放军总医院骨科,北京100853

出　　处：《细胞与分子免疫学杂志》2016年第1期29-33,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81371976;81101387)

摘　　要：目的探究隐丹参酮(CTS)对U2OS骨肉瘤细胞株血管内皮生长因子(VEGF)的调控作用以及对血管生成的影响。方法体外培养的U2OS骨肉瘤细胞分为CTS处理组和对照组,处理组给予(20、40、80、160)μmol/L CTS,对照组给予等体积培养液。实时荧光定量PCR检测U2OS细胞VEGF mRNA的变化,Western blot法和免疫荧光细胞化学染色技术检测VEGF蛋白表达,CCK-8法检测CTS对细胞生长的影响,体外成管实验观察血管形成情况。结果 CTS可明显抑制U2OS细胞内VEGF mRNA和蛋白的表达,且该抑制效应具有剂量依赖性。CCK-8法实验结果显示CTS能抑制U2OS细胞的生长。体外成管实验结果证明CTS对新生血管的形成有抑制作用。结论 CTS可下调U2OS骨肉瘤细胞VEGF的水平,并能抑制血管的生成。Objective To explore the effect of cryptotanshinone（ CTS） on vascular endothelial growth factor（ VEGF）expression in U2 OS osteosarcoma cells,with a focus on angiogenesis. Methods U2 OS osteosarcoma cells cultured in vitro were divided into（ 20,40,80,160） μmol / L CTS treated groups and control group. Real-time quantitative PCR was performed to detect the expression of VEGF mRNA in U2 OS osteosarcoma cells. The VEGF protein level was determined using Western blotting and immunofluorescence cytochemistry. Cell proliferation ability was detected by CCK-8 assay. The tube formation assay in vitro was used to observe the angiogenesis ability. Results CTS inhibited the levels of VEGF mRNA and protein in a dose-dependent manner in U2 OS osteosarcoma cells obviously. CCK-8 assay indicated that CTS inhibited the proliferation of U2 OS osteosarcoma cells. The tube formation assay in vitro revealed that CTS inhibited the angiogenesis ability. Conclusion CTS could down-regulate the expression of VEGF and inhibit angiogenesis in U2 OS osteosarcoma cells.

关 键 词：隐丹参酮 骨肉瘤 血管生成 血管内皮生长因子 

分 类 号：R285.5[医药卫生—中药学] R392.33[医药卫生—中医学]