HPLC-ELSD法测定白果中银杏内酯A、银杏内酯B和银杏内酯C  

Determination of ginkgolide A, ginkgolide B, and ginkgolide C in Ginkgo Semen by HPLC-ELSD

作　　者：秦建平[1,2] 吴建雄[1,2] 潘有智[1,2] 黄文哲[1,2] 王振中[1,2] 萧伟[1,2] 

机构地区：[1]江苏康缘药业股份有限公司,江苏连云港222001 [2]中药制药过程新技术国家重点实验室,江苏连云港222001

出　　处：《现代药物与临床》2016年第2期155-157,共3页Drugs & Clinic

基　　金：国家重大新药创制科技重大专项(2013ZX09402203)

摘　　要：目的建立HPLC-ELSD法同时测定白果药材中银杏内酯A、银杏内酯B和银杏内酯C 3个萜杏内酯成分。方法采用Agilent 5TC C18色谱柱（250 mm×4.6 mm,5μm）,以甲醇–四氢呋喃–水（25∶10∶65）为流动相,体积流量1 m L/min,柱温30℃,蒸发光散射检测器检测,对照品进样量为10、20μL,供试品溶液进样量为5~20μL。结果银杏内酯A在0.37~5.92μg、银杏内酯B在2.8~44.8μg、银杏内酯C在0.65~10.4μg时线性关系良好。银杏内酯A、银杏内酯B、银杏内酯C平均回收率分别为92.40%、95.03%、91.29%,RSD值分别为2.75%、2.06%、2.88%。结论本法操作简单,重复性好,为白果药材的质量控制提供了依据。Objective To establish an HPLC-ELSD method for the determination of three terpene lactones ginkgolide A, ginkgolide B, and ginkgolide C in Ginkgo Semen. Methods The determination was carried out on Agilent 5TC C18 column（250 mm × 4.6 mm, 5 μm）. The mobile phase consisted of methanol – tetrahydrofuran-water（25∶10∶65）. The column temperature was set at 30 ℃ at a flow rate of 1.0 m L/min. ELSD was used as detector to determine the terpene lactones. The injection volumes were 10 and 20 μL for reference substance solution, and 5 — 20 μL for sample solution. Results The linear ranges of ginkgolide A, ginkgolide B, and ginkgolide C were 0.37 — 5.92 μg, 2.8 — 44.8 μg, and 0.65 — 10.4 μg, respectively. The average recoveries of three components were 92.40%, 95.03%, and 91.29% with RSD 2.75%, 2.06%, and 2.88%, respectively. Conclusion The method is simple and repeatable, which can be used in quantity control for Ginkgo Semen.

关 键 词：白果 银杏内酯A 银杏内酯B 银杏内酯C HPLC-ELSD 

分 类 号：R286.02[医药卫生—中药学]