高压氧暴露对脊髓损伤大鼠 IKK－NF－κB 信号通路的影响  

Effect of hyperbaric oxygen on the signal pathway of IKK-NF-κB in the rat with spinal cord injury

作　　者：李茁[1] 高春锦[1] 刘雪华[1] 梁芳[1] 杨晶[1] 李淑娟[2] 苏庆军[3] 

机构地区：[1]首都医科大学附属朝阳医院高压氧科,北京100020 [2]首都医科大学附属朝阳医院神经内科,北京100020 [3]首都医科大学附属朝阳医院骨科,北京100020

出　　处：《中华航海医学与高气压医学杂志》2015年第6期421-425,共5页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：国家国际科技合作专项项目（2012DFA31240）

摘　　要：目的：观察大鼠脊髓损伤（ spinal cord injury ， SCI ）后抑制性κB激酶（ IKKβ）表达的变化及高压氧（ hyperbaric oxygen ，HBO）暴露对它的影响，探讨HBO暴露调控IKK－NF－κB信号通路、减轻SCI后炎性反应及细胞凋亡的作用机制。方法108只成年SD大鼠，按数字表法随机分为假手术组（SH组）、脊髓损伤组（ SCI组）、脊髓损伤＋高压氧组（ SCI＋HBO组），每组根据观察时间点的不同，再分为12 h和1、2、3、7、14 d 6个亚组，每组6只。应用SCI大鼠功能恢复评分（ BBB评分）在制模后12 h和1、2、3、7、14 d分别观察上述3组相应亚组的后肢功能，采用荧光定量PCR（ RT－PCR）和Western blot法测定IKKβ蛋白及mRNA的含量。结果 SCI后损伤组织IKKβ蛋白的含量除12 h外，其余各时间点均明显增加，与SH组比较差异具有统计学意义（P＜0．01）（造模后1 d：SCI组为0．250±0．043，SCI＋HBO组为0．135±0．023，SH组为0．075±0．006）；与SH组比较，SCI组IKKβmRNA的含量在12 h、1、2、3 d明显升高（P＜0．01）（SH组分别为：0．753±0．275，0．830±0．342，0．895±0．181，0．958±0．528；SCI组分别为：1．868±1．019，3．435±2．282，4．232±3．080，2．777±1．579），在第7天升高（P＜0．05），在第14天2组间差异无统计学意义（P＞0．05）。 HBO暴露后，脊髓IKKβ蛋白的含量在各时间点均较SCI组显著降低，差异具有统计学意义（P＜0．01）；SCI＋HBO组IKKβmRNA的含量在12 h、1、3、7 d时低于SCI组，差异具有统计学意义（P＜0．05或P＜0．01），2 d和14 d 2组间差异无统计学意义（P＞0．05）。与SH组比较，SCI＋HBO组7、14 d BBB评分改善，差异具有统计学意义（ P＜0．05）。结论 SCI后IKKβ蛋白和mRNA的含量升高，HBO暴露可能通过下调IKKβ激酶表达，调控IKK－NF－κB信号通路，减轻SCI后炎性反应及细胞凋亡。Objective To observe the effect of hyperbaric oxygen （HBO） on the expression levels of inhibitory kappa B kinase （ IKKβ） in the rat with spinal cord injury （ SCI ） , and also to investigate the mechanisms that might be involved in its effect on the regulation of the signal pathway of IKK -NF-κB, as well as inflammatory reaction and apoptosis of cells following spinal cord injury .Methods One hundred and eight healthy adult SD rats were randomly divided into 3 groups： the sham surgery group （ or the SS group ） , the spinal cord injury group （or simply the SCI group）, and the SCI ＋HBO group.Then, each group was further subdivided into 6 subgroups, i.e.the 12 h after injury subgroup （or the 12 h subgroup）, the 1 day after injury subgroup （or the 1 d subgroup）, the 2 d subgroup, the 3 d subgroup, the 7 d subgroup and the 14 d subgroup,＆amp;nbsp;each consisting of 6.Functional recovery of the hind limb was assessed by Basso , Beattie and Bresnahan （ BBB） scores at different time points after the development of the SCI model .The expression levels of IKKβand mRNA in the spinal cord were detected by fluorescent RT-PCR and Western blot .Results The expression levels of IKKβin the damaged tissues were all significantly increased except at hour 12 after injury, and statistical significance could be seen , as compared with those of the SH group （P〈0.01）, for example on day 1, the SCI group：0.250 ±0.043;the SCI＋HBO group：0.135 ±0.023; the SH group：0.075 ±0.006.The expression levels of IKKβmRNA were significantly increased at hour 12, day 1, day 2 and day 3 （P〈0.01 or P〈0.05）（the SCI group：1.868 ±1.019,3.435 ±2.282,4.23 ±3.070,2.777 ±1.579;the SH group：0.753 ±0.275, 0.830 ±0.342,0.895 ±0.181,0.958 ±0.528）.There were no obvious differences in its expression levels, when comparisons were made between the SH group and the SCI group （P 〉0.05）.After HBO exposure , the expression levels of IKKβin the spinal cord at various time points were al

关 键 词：高压氧 脊髓损伤 抑制性κB激酶(IKKβ) 大鼠 

分 类 号：R651.2[医药卫生—外科学] R459.6[医药卫生—临床医学]