纯镁超声微弧氧化-植酸-丝素复合处理涂层的细胞生物相容性  

The improved cell-biocompatibility of pure magnesium-ultrasonic micro-arc oxidation(MAO)-phytic acid-fibroin treated composite coating

作　　者：刘继光[1] 王爽[1] 李慕勤[1] 魏方红[1] 陈洪伟[1] 刘明达[1] 

机构地区：[1]佳木斯大学黑龙江省高校生物医学材料重点实验室,黑龙江佳木斯154007

出　　处：《中国体视学与图像分析》2015年第4期384-391,共8页Chinese Journal of Stereology and Image Analysis

基　　金：国家自然科学基金(No.31370979);黑龙江省高校创新团队建设计划项目(2012TD010);佳木斯大学研究生科技创新项目(LZZ2014_024)

摘　　要：目的通过对纯镁超声微弧氧化表面进行植酸及丝素的复合处理,调控纯镁超声微弧氧化的耐腐蚀性和提高细胞生物相容性。方法实验分为三组,A组纯镁超声微弧氧化,B组纯镁超声微弧氧化-植酸,C组纯镁超声微弧氧化-植酸-丝素。通过SEM扫描电镜观察表面形貌,CCK-8(Cell Counting Kit-8)检测细胞增殖和粘附能力,通过激光共聚焦显微镜检测细胞粘附形态,ALP(碱性磷酸酶)检测细胞碱性磷酸酶活性。结果 B组纯镁MAO-植酸处理后,与A组纯镁MAO表面形态比较,减少了膜层微孔和裂纹,由于丝素蛋白与植酸的羟基发生了氢键及配位键作用,使C组纯镁MAO植酸-丝素较A、B两组更易于成膜,增加生物活性。结论经过超声微弧氧化-植酸-丝素复合处理后的试件,其生物相容性明显提升,优于其他两组。Objective Through the composite treatment of pure magnesium surface, regulating the corro-sion of pure magnesiumby uhrasonicmicro-arc oxidation broin,to improveits cell biocompatibility. Methods （MAO） , and treated with phytic acid and silk fi- The experimental subjects were divided into, three groups The group A was treated with pure magnesium MAO, the group B was treated by pure magnesium MAO and phytic acid. The group C was treated with pure magnesium MAO, phytic acid and silk fibroin. The surface morphology was observed by scanning electron microscopy. The cell proliferation ability was de- tected by cell counting CCK-8 kit, the adhesion ability was detected by confocal laser scanning microscopy and the cell activity was detected by alkaline phosphatase （ALP） assay~ Results The MAO layer of pure magnesium of the group B was treated by phytic acid pared with those of the group A. Because of the effect ~ The membrane pores and cracks were reduced com- of coordination bond and hydroxyl hydrogen bonding, asurface membrane was more easily to be formed with silk fibroin and phytic acid, thus increasing the cell biological activ silk fibroin, th ity. eb Conclusions After the complex treatment by ultrasonic micro-arc oxidation-phytic acid- iological compatibility of the test piecesis significantly improved, compared with those of other two groups.

关 键 词：纯镁 超声微弧氧化 植酸 丝素 细胞相容性 

分 类 号：R318.08[医药卫生—生物医学工程]