高功率微波致大鼠卵巢损伤的病理形态学和定量研究  

The pathological morphology and quantitative study of damages in the rats' ovaries induced by high-power microwave

作　　者：谢一帆[1] 刘肖[1] 吴琼[2] 崔亮[1] 徐新萍[1] 王少霞[1] 张翠芳[1] 吴小红[1] 严格[1] 张潇[1] 苏慧婷[1] 左红艳[1] 王德文[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850 [2]军事医学科学院附属医院妇科,北京100071

出　　处：《中国体视学与图像分析》2015年第4期424-433,共10页Chinese Journal of Stereology and Image Analysis

基　　金：国家自然科学基金面上项目(81472951);公安部重点攻关计划项目(公科研[2008]208号)

摘　　要：目的探讨高功率微波导致卵巢组织形态学改变的规律及其特点。方法雌性Wistar大鼠60只,随机分为对照组(C)和照射组(R),照射组大鼠经平均功率密度100 m W/cm2的S-波段HPM辐照15 min,分别于辐照后1 d、3 d、7 d、14 d、28 d和3 m活杀,取双侧卵巢,制作石蜡切片和超薄切片,光镜和电镜下观察组织形态学改变,并分别对卵泡和黄体数量进行定量计数。结果照射后卵巢组织结构发生明显损伤:原始闭锁卵泡和囊状闭锁卵泡数量持续性增多(P<0.05),囊状卵泡存活率显著降低(P<0.05);黄体数量进行性增多(P<0.05),颗粒黄体细胞凋亡增多;其中,囊状卵泡、颗粒细胞和颗粒黄体细胞损伤尤为严重。结论高功率微波可导致大鼠卵巢组织形态学改变,具有广泛性、速发性、持续性和不同卵泡与细胞敏感程度差异性特点。Objective To explore the regularities and characteristics of the histomorphological changes in the rats＇ ovary induced by high-power microwave. Methods Sixty female Wistar ratswere randomly divid- ed into the control group （C） and the irradiation group （R）. Wistar rats of the irradiation group were irradi- ated by S-HPM of the average power density 100 mW/cm2 for 15 minutes. The rats were killed at l d,3 d, 7 d,14 d,28 d and 3 m after irradiation. Bilateral ovaries were collected. The paraffin ob served under a light microscope and an electron microscope. All the follicles and the corpora lutea were classified and counted under a light microscope. Results The histological appearance of ovaries happened the obvious damage after irradiation. The number of primordial atretic follicles and antral atretic follicles increased sus- tainability （P 〈 0.05 ）. The survival rate of the antral follicles were significantly decreased （P 〈 0.05）. The number of corpus luteum increased progressively（P 〈 0.05）. The number of apoptosis in the granulosa lu- tein cells increased. The worse damages happened in the antral follicles, granulosa cells and granulosa lute- in cells. Conclusions HPM can cause histomorphological changes of rats＇ ovaries. The worst damage hap- pens in the antral follicles. The characteristics are universality, speedy and sustainability. It is significantly differences of the sensitive degree in different follicles and cells.

关 键 词：高功率微波 卵巢 卵泡 

分 类 号：R594.8[医药卫生—内科学]