超声微泡介导VEGF基因转染治疗缺血性疾病研究进展  

Progressin Ultrasound Microbubble Mediated Vascular Endothelial Growth Factor Gene Expression for Treatment of Ischemic Disease

作　　者：范春艳[1] 徐春媚[1] 王璐璐[1] 毕伟[1] 薛晓蕊 

机构地区：[1]哈尔滨医科大学附属第四医院,黑龙江哈尔滨150001

出　　处：《现代生物医学进展》2015年第35期6990-6993,共4页Progress in Modern Biomedicine

基　　金：黑龙江省青年科学基金项目(QC2010090)

摘　　要："治疗性血管新生"是利用外源性血管生长因子或基因促进缺血部位新生血管形成,达到改善缺血部位血液供应而起到治疗的目的,该方法为缺血性疾病的治疗提供了新的思路。目前研究的多种与血管生成相关的因子中,血管内皮生长因子(Vascular Endothelial Growth Factor,VEGF)是公认的最具特异性且作用最强的促进血管生长因子。但由于外源性血管生长因子重组蛋白在体内半衰期短,试验中难以长时间持续给药起到刺激新血管生成及成熟的作用。研究表明通过超声破坏微泡技术可使基因转染的靶细胞持续表达该基因。因此,应用超声靶向微泡破坏技术使VEGF基因在缺血部位持续表达,可起到治疗性血管新生的作用。本文将就超声微泡介导VEGF基因转染治疗缺血性疾病研究进展进行综述。Therapeutic neovascularization is a new concept for the treatment of ischemic vascular diseases. This method can improve the blood supply of ischemic area and achieve the purpose of treatment by using exogenous vascular growth factor or gene to promote angiogenesis in ischemic region. Among the several angiogenic growth factors, Vascular Endothelial Growth Factor （VEGF） has been shown to have a key rolewith stimulating angiogenesis. However the process of growing mature vessels takes time. This time course poses a significant problem for administration of recombinant VEGF protein, as the half life of VEGF is so short in plasma. Assuming that VEGF needs to be present throughout the creation of new vessels, but this would be virtually impossible to achieve in a clinical setting by using the recombinant protein. Gene and stem cell based therapy represents ways by which elevated levels of VEGF can be produced and sustained for longer period of time. Ultrasound-targeted microbubble destruction （UTMD） is a new strategy for targeting VEGF gene delivery and can make the gene expressed in a long time. This articlereviewed progress inVEGF gene transfection for treatent of ischemicdiseases.

关 键 词：超声微泡 血管内皮生长因子 治疗性血管新生 缺血性疾病 

分 类 号：Q681[生物学—生物物理学]