髓系抑制性细胞在慢性乙型肝炎中的表达特点及与肝病相关性研究  

Expression features of CD14^+ HLA-DR^(-/low) myeloid-derived suppressor cells in chronic hepatitis B patients and its correlation with liver diseases

作　　者：黄昂[1] 闫伟伟[1] 张波[2] 孙颖[1] 滕光菊[1] 李保森[1] 樊克兴[2] 邹正升[3] 

机构地区：[1]解放军第三〇二医院非感染性肝病诊疗与研究中心,北京100039 [2]解放军总医院肿瘤中心 [3]北京解放军第三〇二医院非感染性肝病诊疗与研究中心,100039

出　　处：《肝脏》2015年第11期844-846,共3页Chinese Hepatology

摘　　要：目的观察不同人群和不同感染时期的慢性乙型肝炎患者外周血中CD14^+ HLA-DR^(-/low)髓系抑制性细胞(MDSC)频率、表型变化以及与疾病进程之间的关系。方法利用荧光抗体标记结合多色流式技术,检测慢性乙型肝炎(CHB)患者外周血CD14^+ HLA-DR^(-/low)细胞频率及表面PD-L1/PD-1分子的表达。结果CHB患者外周血的CD14^+ HLA-DR^(-/low)频率明显高于健康对照;CHB患者CD14+HLA-DR-/lo频率与ALT呈负相关(R=-0.285,P=0.01),与HBV DNA载量成正相关(R=0.396,P=0.01);CHB患者外周血CD14^+ HLA-DR^(-/low)细胞上PD-1的表达高于CD14+HLA-DR+单核细胞。结论 CD14^+ HLA-DR^(-/low)细胞可能与HBV感染慢性化相关。Objective To investigate the frequency and phenotype modulation of CD14 ^＋ HLA-DR^-/low myeloid-derived suppressor cells （MDSCs ）in different populations and phases of chronic hepatitis B （CHB）,and to explore their relationship with disease progression.Methods Immunofluorescent staining and flow cytometry assay were performed to measure frequency and expression of PD-L1/PD-1 in CD14 ^＋ HLA-DR^-/low MDSCs for CHB patients.Results In CHB patients,frequency and expression of PD-L1/PD-1 in peripheral CD14 ^＋ HLA-DR^-/low cells were significantly higher than those in healthy controls,respectively.Additionally,frequency of circulating CD14 ^＋ HLA-DR^-/low cells were negatively correlated with alanine aminotransferase levels（R=-0.285,P =0.01 ）,while positively correlated with HBV DNA loads （R=0.396,P =0.01）.Conclusion CD14 ^＋ HLA-DR^-/low MDSCs might be closely related to chronicity of HBV infection.

关 键 词：慢性乙型肝炎 CD14 HLA-DR 髓系抑制性细胞 

分 类 号：R512.62[医药卫生—内科学]