辅助生殖技术中锌抑制精子氧化损伤作用  

Zinc protects sperm from being damaged by reactive oxygen species in assisted reproductive technology

作　　者：陈芦地[1] 吴金香[1] 王正尧[1] 谢远志[2] 

机构地区：[1]福建医科大学附属第二医院检验科,泉州362000 [2]福建医科大学附属第二医院生殖男科

出　　处：《中国男科学杂志》2015年第10期8-11,共4页Chinese Journal of Andrology

基　　金：福建省卫生厅青年科研基金资助项目(NO.2011-1-36)~~

摘　　要：目的：探讨辅助生殖技术中锌对精子损伤的保护作用。方法以辅助生殖技术中常用的密度梯度离心法优选后的精子为研究对象，以过氧化氢为损伤刺激物，应用国联精子检测系统、光学显微镜以及精子DNA碎片分析试剂盒的方法比较精子的活力与存活率，精子质膜完整性以及精子DNA碎片形成，从而分析锌抑制过氧化氢对精子的损伤作用。结果过氧化氢刺激后精子的活动力与存活率差，精子质膜完整性破坏，大量精子DNA损伤，而锌能明显保护精子的损伤作用。结论精子损伤与氧化刺激密切相关，在辅助生殖技术中锌可抑制精子的损伤作用。Objective To explore the protective action of zinc insperm damage during assisted reproductive technology.Methods Preferring the sperm samples which were selected by density gradient centrifugation in assisted reproductive technology were used for the study. Uder stimulation of hydrogen peroxide, sperm motility, viability, sperm membrane integrity and DNA fragmentation were detected by Goodline sperm detection system, optical microscopy and sperm DNA fragmentation assay kit respectively, and the effect of Zinc on sperm damage from hydrogen peroxide was evaluated.Results The motility and vitality of sperms were decreased, the membrane integrity was destroyed and lots of sperm DNA were damaged under hydrogen peroxide stimulation. However, Zinc could protect sperm from damage obviously.Conclusion Sperm injury is closely associated with oxidative stimulation. Zinc could induce sperm damage in assisted reproductive technology.

关 键 词：精子 活性氧 生殖技术 辅助 锌 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]