清浊祛毒丸联合针刺对实验性大鼠慢性非细菌性前列腺炎的治疗作用  

The effects of Qingzhuo Qudu Wan combined with acupuncture on experimental rats with chronic nonbacterial prostatitis

作　　者：李世林[1] 唐梁[2] 叶海霞[3] 蒲小勇[4] 王怀鹏[4] 

机构地区：[1]广州市第一人民医院南沙医院泌尿外科,广州511457 [2]广州市第一人民医院南沙医院中医科 [3]广州市第一人民医院南沙医院神经内科 [4]广东省人民医院泌尿外科

出　　处：《中国男科学杂志》2015年第11期35-40,共6页Chinese Journal of Andrology

基　　金：广东省中医药管理局中医药课题项目(NO.20132002)

摘　　要：目的探讨清浊祛毒丸联合针刺对实验性大鼠慢性非细菌性前列腺炎（CNP）的治疗作用及分子机制。方法选择10月龄健康雄性Wistar大鼠72只，随机分为正常对照组、安慰剂组、西药组、中药组、针刺组及联合治疗组（简称联合组），每组各12只。采用去势加大鼠后背皮下注射17B雌二醇（17β-E2）制作CNP模型，造模成功后除正常对照组及安慰剂组给予生理盐水治疗外，其他各组分别予坦索罗辛加塞来昔布胶囊、清浊祛毒丸、针刺、清浊祛毒丸加针刺治疗。治疗周期为30d。治疗结束后处死大鼠，显微镜下观察大鼠前列腺组织病变情况，采用ELISA法检测前列腺液中IL-1β／TNF-α的表达水平，Westernblot检测MIF蛋白的表达情况，统计分析各组指标的变化差异。结果实验各组均成功造模，6组在前列腺组织炎症程度、前列腺液中IL-1β／TNFα的表达水平、前列腺组织中MIF蛋白的表达水平等方面差异均有显著性意义（P〈0．05）；各治疗组与安慰剂组比较各项指标均有不同程度的改善，各组间比较差异均有显著性意义（P〈0．05）；治疗组间比较显示，联合组大鼠前列腺组织炎症程度较其他三组明显减轻，且IL-1β／TNFα及MIF的表达水平也明显降低，两两比较差异有统计学意义（P〈0．05）。结论清浊祛毒丸联合针刺对实验性大鼠慢性非细菌性前列腺炎有明显的治疗作用，可减轻前列腺炎症程度，降低炎性细胞因子的表达，是治疗慢性非细菌性前列腺炎的一种可行方法。Objective To investigate the effects of Qingzhuo Qudu Wan combined with acupuncture on experimental rats with chronic nonbacterial prostatitis （CNP） and discuss its molecular mechanisms. Methods Total of 72 10-month-old healthy male Wistar rats were randomly divided into the control group, the placebo group, western medicine group, TCM group, acupuncture group and combined treatment group （combined group）, each group 12 rats. Rat model with CNP was induced by castration and injecting 17 β -estradiol （17 β -E2） production into subcutaneous of rats＇ back. After the models were successfully made, rats in the normal control group and the placebo group received saline treatment, rats in the other groups were treated with tamsulosin and celecoxib capsules, Qingzhuo Qudu Wan, acupuncture, Qingzhuo Qudu Wan plus acupuncture respectively. Treatment period was 30 days. After treatment, the rats were sacrificed and the lesions of rat prostate tissue were observed under a microscope, the expression levels of IL- 1β / TNF-α in prostatic fluid were detected by ELISA and the expression levels of MIF in prostate tissue were detected by Western Blot. The differences of indexes among groups were analyzed by statistical analysis. Results The models were successfully established in each group. There were significant differences in prostate tissue inflammation, level of prostatic fluid IL- 1β/TNF- α or expression of MIF in prostate tissue among six groups （P〈0.05）; Compared with those of the placebo group, all indexes in each treatment group were all improved in varying degrees, differences among groups were statistically significant （P〈0.05）; Compared with that of the other three groups among the treatment groups, rat prostate tissue inflammation of the combined group was significantly decreased, and the expression level of IL-1 β / TNF- α / MIF was significantly reduced, differences among the two groups were statistically significant （P〈0.05）. Conclusion Qingzhuo Qudu Wan combined with acupun

关 键 词：清浊祛毒丸 针刺 前列腺炎 大鼠 

分 类 号：R697.33[医药卫生—泌尿科学]